Bioactive grade

Recombinant human Ephrin A1 protein (Fc Chimera) (ab181919)

Description

  • Product name

    Recombinant human Ephrin A1 protein (Fc Chimera)
    See all Ephrin A1 proteins and peptides
  • Biological activity

    Measured by its binding ability in a functional ELISA. When Recombinant Human EphA2 is coated at 4 µg/ml (100 µl/well), the concentration of ab181919 that produces 50% of the optimal binding response is found to be approximately 0.5 - 4 ng/ml.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      DR HTVFWNSSNP KFRNEDYTIH VQLNDYVDII CPHYEDHSVA DAAMEQYILY LVEHEEYQLC QPQSKDQVRW QCNRPSAKHG PEKLSEKFQR FTPFTLGKEF KEGHSYYYIS KPIHQHEDRC LRLKVTVSGK ITHSPQAHDN PQEKRLAADD PEVRVLHSIG HS
    • Predicted molecular weight

      46 kDa
    • Amino acids

      19 to 182
    • Additional sequence information

      Mature protein fused with Fc fragment of human IgG1 at the C-terminus.

Specifications

Our Abpromise guarantee covers the use of ab181919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C prior to reconstitution. Upon delivery aliquot. Store at -80°C. Reconstitute for long term storage.

    pH: 7.50
    Constituents: 0.75% Glycine, 5% Trehalose, 0.61% Tris

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in 100 µl sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • B61
    • ECKLG
    • EFL 1
    • EFL1
    • EFNA 1
    • Efna1
    • EFNA1_HUMAN
    • EPH related receptor tyrosine kinase ligand 1
    • EPH-related receptor tyrosine kinase ligand 1
    • Ephrin-A1
    • Ephrin-A1, secreted form
    • EphrinA1
    • EPLG 1
    • EPLG1
    • Immediate early response protein B61
    • LERK 1
    • LERK-1
    • LERK1
    • Ligand of eph related kinase 1
    • OTTHUMP00000033242
    • OTTHUMP00000033271
    • secreted form
    • TNF alpha-induced protein 4
    • TNFAIP 4
    • TNFAIP4
    • Tumor necrosis factor alpha induced protein 4
    • Tumor necrosis factor alpha-induced protein 4
    see all
  • Function

    Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cones.
  • Tissue specificity

    Brain. Down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells (at protein level).
  • Sequence similarities

    Belongs to the ephrin family.
  • Post-translational
    modifications

    Undergo proteolysis by a metalloprotease to give rise to a soluble monomeric form.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

Images

  • Human Ephrin-A1 (Fc Tag) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
  • SDS-PAGE analysis of ab181919 as determined by DTT-reduced (+) and non-reduced (-) SDS-PAGE and staining overnight with Coomassie Blue.

References

ab181919 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181919.
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