Recombinant Human EPO protein (ab155618)

Submit a review Q&A (1)
SDS-PAGE - Recombinant Human EPO protein (ab155618)

    Key features and details

    • Expression system: HEK 293 cells
    • Purity: > 97% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human EPO protein
      See all EPO proteins and peptides

    • Purity

      > 97 % SDS-PAGE.

    • Endotoxin level

      < 1.000 Eu/µg

    • Expression system

      HEK 293 cells

    • Accession

      P01588

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        APPRLICDSRVLERYLLEAKEAENITTGCAEHCSLNENITVPDTKVNFYA WKRMEVGQQAVEVWQGLALLSEAVLRGQALLVNSSQPWEPLQLHVDKAVS GLRSLTTLLRALGAQKEAISPPDAASAAPLRTITADTFRKLFRVYSNFLR GKLKLYTGEACRTGDR

      • Predicted molecular weight

        18 kDa

      • Amino acids

        28 to 193

    Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab155618 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        SDS-PAGE

      • Form

        Lyophilized
      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C. Avoid freeze / thaw cycle.

        pH: 7.40
        Constituents: 95% PBS, 5% Trehalose

      • Reconstitution
        Reconstitute with sterile deionized water to a concentration of 400 µg/ml.

      General Info

      • Alternative names

        • EP
        • EPO
        • EPO alpha
        • Epoetin
        • Erythropoetin
        • Erythropoietin precursor
        • MVCD2
        see all

      • Relevance

        Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

      • Cellular localization

        Secreted

      Images

      • SDS-PAGE - Recombinant Human EPO protein (ab155618)
        SDS-PAGE - Recombinant Human EPO protein (ab155618)

        SDS-PAGE analysis of reduced ab155618 stained overnight with Coomassie Blue. The protein has a calculated MW of 18.4 kDa. The protein migrates as 28-35 kDa under reducing (Lane 1) condition due to glycosylation.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab155618? Please let us know so that we can cite the reference in this datasheet.

      ab155618 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Would you be able to let me know the biological activity of the current batch of this and also whether it is in stock?
      Additionally, is this the correct EPO version if wanting to administer it to mice via intra-peritoneal injection to assess neurogenesis?
      I actually need 31 500IU of EPO so I'm not sure if the 10ug size will be enough?

      Read More
      Answer


      The quality control criteria for bioactivity of EPO is actually no less than 1.5x10e5 IU/mg.
      So If customer need 31,500 IU totally, around 210ug is required. Our recommendation is to order 5 vials x50ug to have some safety margin for this case.
      EPO product ab155618 is ready for any in vivo experiments as an active protein.
      We believe that EPO has been injected successfully to mice via various methods, including intra-peritoneal injection, but we are not sure if neurogenesis will occur as expected.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com