Recombinant Human EPO protein (ab155618)

Submit a review Q&A (1)

Description

  • Product name

    Recombinant Human EPO protein
    See all EPO proteins and peptides

  • Purity

    > 97 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    P01588

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      APPRLICDSRVLERYLLEAKEAENITTGCAEHCSLNENITVPDTKVNFYA WKRMEVGQQAVEVWQGLALLSEAVLRGQALLVNSSQPWEPLQLHVDKAVS GLRSLTTLLRALGAQKEAISPPDAASAAPLRTITADTFRKLFRVYSNFLR GKLKLYTGEACRTGDR

    • Predicted molecular weight

      18 kDa

    • Amino acids

      28 to 193

Specifications

Our Abpromise guarantee covers the use of ab155618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 95% PBS, 5% Trehalose

  • Reconstitution
    Reconstitute with sterile deionized water to a concentration of 400 µg/ml.

General Info

  • Alternative names

    • EP
    • EPO
    • EPO alpha
    • Epoetin
    • Erythropoetin
    • Erythropoietin precursor
    • MVCD2
    see all

  • Relevance

    Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

  • Cellular localization

    Secreted

Images

  • SDS-PAGE - Recombinant human EPO protein (ab155618)
    SDS-PAGE - Recombinant human EPO protein (ab155618)
    Purity of ab155618 determined by SDS-PAGE analysis under reduced conditions, and stained overnight with Coomassie Blue.

References

ab155618 has not yet been referenced specifically in any publications.

Publishing research using ab155618? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Would you be able to let me know the biological activity of the current batch of this and also whether it is in stock?
Additionally, is this the correct EPO version if wanting to administer it to mice via intra-peritoneal injection to assess neurogenesis?
I actually need 31 500IU of EPO so I'm not sure if the 10ug size will be enough?

Read More
Answer


The quality control criteria for bioactivity of EPO is actually no less than 1.5x10e5 IU/mg.
So If customer need 31,500 IU totally, around 210ug is required. Our recommendation is to order 5 vials x50ug to have some safety margin for this case.
EPO product ab155618 is ready for any in vivo experiments as an active protein.
We believe that EPO has been injected successfully to mice via various methods, including intra-peritoneal injection, but we are not sure if neurogenesis will occur as expected.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com