Recombinant human EPO protein (Active) (ab208463)
Key features and details
- Expression system: Insect cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human EPO protein (Active)
See all EPO proteins and peptides -
Biological activity
Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is ≤ 0.5 ng/ml.
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Purity
> 95 % SDS-PAGE.
ab208463 was purified using conventional chromatography. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Insect cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALRAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDRLEHH HHHH -
Predicted molecular weight
20 kDa including tags -
Amino acids
28 to 193 -
Tags
His tag C-Terminus -
Additional sequence information
This product is for the mature full length protein. The signal peptide is not included. NCBI Accession No.: NP_00079. SDS–PAGE under reducing conditions the protein migrates to 28-40 KDa
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab208463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- EP
- EPO
- EPO alpha
see all -
Relevance
Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. -
Cellular localization
Secreted
Images
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SDS-PAGE - Recombinant human EPO protein (Active) (ab208463)
15% SDS-PAGE analysis of ab208463 (3 µg)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab208463 has not yet been referenced specifically in any publications.
