Product nameRecombinant Human Epsin 1 protein
See all Epsin 1 proteins and peptides
Protein lengthProtein fragment
Amino Acid Sequence
Amino acids346 to 428
Tagsproprietary tag N-Terminus
Our Abpromise guarantee covers the use of ab162266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesProtein concentration is above or equal to 0.05 mg/ml.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.79% Tris HCl
- EH domain binding mitotic phosphoprotein
- EH domain-binding mitotic phosphoprotein
- EPN 1
FunctionBinds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.
Sequence similaritiesBelongs to the epsin family.
Contains 1 ENTH (epsin N-terminal homology) domain.
Contains 3 UIM (ubiquitin-interacting motif) repeats.
DomainThe NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP2A2 and clathrin binding.
The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction with the AP-2 complex subunit AP2B1.
modificationsPhosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.
Cellular localizationCytoplasm. Cell membrane. Nucleus. Membrane > clathrin-coated pit. Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145.
- Information by UniProt
ab162266 has not yet been referenced specifically in any publications.