Recombinant Human eRF3/GSPT1 protein (ab132287)

Submit a review Submit a question
SDS-PAGE - Recombinant Human eRF3/GSPT1 protein (ab132287)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: ELISA, SDS-PAGE, WB

    Description

    • Product name

      Recombinant Human eRF3/GSPT1 protein

    • Expression system

      Wheat germ

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MDPGSGGGGGGGGSSSGSSSSDSAPDCWDQADMEAPGPGPCGGGGSLAAA AEAQRENLSAAFSRQLNVNAKPFVPNVHAAEFVPSFLRCPAAPPPPAGGA ANNHGAGSGAGGRAAPVESSQEEQSLCEGSNSAVSMELSEPIENGETEMS PEESWEHKEEISEAEPGGGSLGDGRPPEESAHEMMEEEEEIPKPKSVVAP PGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEK NRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFV PNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLI VLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTG ANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTV VLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRL KGIEEEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHI HTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLE TFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD

      • Predicted molecular weight

        95 kDa including tags

      • Amino acids

        1 to 633

    Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab132287 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      SDS-PAGE

      Western blot

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • 551G9.2
      • Anti Eukaryotic Release Factor 3a
      • Anti G1 to S phase transition 1
      • Anti GST1, homolog of yeast
      • eRF 3a
      • eRF3a
      • G1 to S phase transition 1
      • G1 to S phase transition protein
      • G1 to S phase transition protein 1 homolog
      • GSPT 1
      • GSPT1
      • GST 1
      • GST1
      • GTP binding protein GST1 HS
      • Transition protein 1
      see all

    • Relevance

      Translation termination in eukaryotes is governed by two proteins, belonging to the class-1 (eRF1) and class-2 (eRF3) polypeptide release factors. eRF3/GSPT1 is involved in regulation of mammalian cell growth and belongs to the GTP-binding elongation factor family (ERF3 subfamily). eRF3 catalyzes hydrolysis of GTP to GDP and inorganic phosphate in the ribosome in the absence of mRNA, tRNA, aminoacyl-tRNA and peptidyl-tRNA.

    • Cellular localization

      Cytoplasmic

    Images

    • SDS-PAGE - Recombinant Human eRF3/GSPT1 protein (ab132287)
      SDS-PAGE - Recombinant Human eRF3/GSPT1 protein (ab132287)
      12.5% SDS-PAGE stained with Coomassie Blue showing ab132287.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab132287? Please let us know so that we can cite the reference in this datasheet.

    ab132287 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab132287.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com