Recombinant Human eRF3/GSPT1 protein (ab132287)
Key features and details
- Expression system: Wheat germ
- Suitable for: ELISA, SDS-PAGE, WB
Description
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Product name
Recombinant Human eRF3/GSPT1 protein -
Expression system
Wheat germ -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MDPGSGGGGGGGGSSSGSSSSDSAPDCWDQADMEAPGPGPCGGGGSLAAA AEAQRENLSAAFSRQLNVNAKPFVPNVHAAEFVPSFLRCPAAPPPPAGGA ANNHGAGSGAGGRAAPVESSQEEQSLCEGSNSAVSMELSEPIENGETEMS PEESWEHKEEISEAEPGGGSLGDGRPPEESAHEMMEEEEEIPKPKSVVAP PGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEK NRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFV PNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLI VLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTG ANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTV VLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRL KGIEEEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHI HTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLE TFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD -
Predicted molecular weight
95 kDa including tags -
Amino acids
1 to 633
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab132287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
SDS-PAGE
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
General Info
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Alternative names
- 551G9.2
- Anti Eukaryotic Release Factor 3a
- Anti G1 to S phase transition 1
see all -
Relevance
Translation termination in eukaryotes is governed by two proteins, belonging to the class-1 (eRF1) and class-2 (eRF3) polypeptide release factors. eRF3/GSPT1 is involved in regulation of mammalian cell growth and belongs to the GTP-binding elongation factor family (ERF3 subfamily). eRF3 catalyzes hydrolysis of GTP to GDP and inorganic phosphate in the ribosome in the absence of mRNA, tRNA, aminoacyl-tRNA and peptidyl-tRNA. -
Cellular localization
Cytoplasmic
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab132287 has not yet been referenced specifically in any publications.