Overview

  • Product name

    Recombinant human ERK5 protein
  • Protein length

    Full length protein

Description

  • Nature

    Recombinant
  • Source

    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Molecular weight

      132 kDa including tags
    • Amino acids

      1 to 816

Associated products

Specifications

Our Abpromise guarantee covers the use of ab126913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The specific activity of ab126913 was determined to be 11 nmol/min/mg
  • Applications

    Functional Studies

    Western blot

    SDS-PAGE

  • Purity

    > 75 % Densitometry.
    The purity was determined to be >75% by densitometry
  • Form

    Liquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Big MAP kinase 1
    • BMK 1
    • BMK 1 kinase
    • BMK-1
    • BMK1
    • BMK1 Kinase
    • EC 2.7.11.24
    • ERK 4
    • ERK 5
    • ERK-5
    • ERK4
    • ERK5
    • Extracellular signal regulated kinase 5
    • Extracellular signal-regulated kinase 5
    • MAP kinase 7
    • MAPK 7
    • MAPK7
    • Mitogen activated protein kinase 7
    • Mitogen-activated protein kinase 7
    • MK07_HUMAN
    • OTTHUMP00000065906
    • OTTHUMP00000065907
    • PRKM 7
    • PRKM7
    • PROTEIN KINASE, MITOGEN-ACTIVATED, 7
    see all
  • Function

    Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways.
  • Tissue specificity

    Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
  • Cellular localization

    Cytoplasm. Nucleus. Translocates to the nucleus upon activation.
  • Information by UniProt

Images

  • SDS-PAGE using ab126913
  • The specific activity of ab126913 was determined to be 11 nmol/min/mg

References

This product has been referenced in:

  • Liu W  et al. Metabolic stress-induced cardiomyopathy is caused by mitochondrial dysfunction due to attenuated Erk5 signaling. Nat Commun 8:494 (2017). Read more (PubMed: 28887535) »
See 1 Publication for this product

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