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    recombinant-human-erp57-protein-ab92937.pdf

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Signal Transduction Protein Trafficking ER Proteins
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Recombinant Human ERp57 protein (ab92937)

  • Datasheet
Reviews (1)Q&A (1)

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SDS-PAGE - Recombinant Human ERp57 protein (ab92937)
  • Western blot - Recombinant Human ERp57 protein (ab92937)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Tags: His tag N-Terminus
  • Suitable for: WB, SDS-PAGE

Description

  • Product name

    Recombinant Human ERp57 protein
    See all ERp57 proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    ab92937 is purified using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Accession

    P30101
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSDV LELTDDNFES RISDTGSAGL MLVEFFAPWC GHCKRLAPEY EAAATRLKGI VPLAKVDCTA NTNTCNKYGV SGYPTLKIFR DGEEAGAYDG PRTADGIVSH LKKQAGPASV PLRTEEEFKK FISDKDASIV GFFDDSFSEA HSEFLKAASN LRDNYRFAHT NVESLVNEYD DNGEGIILFR PSHLTNKFED KTVAYTEQKM TSGKIKKFIQ ENIFGICPHM TEDNKDLIQG KDLLIAYYDV DYEKNAKGSN YWRNRVMMVA KKFLDAGHKL NFAVASRKTF SHELSDFGLE STAGEIPVVA IRTAKGEKFV MQEEFSRDGK ALERFLQDYF DGNLKRYLKS EPIPESNDGP VKVVVAENFD EIVNNENKDV LIEFYAPWCG HCKNLEPKYK ELGEKLSKDP NIVIAKMDAT ANDVPSPYEV RGFPTIYFSP ANKKLNPKKY EGGRELSDFI SYLQREATNP PVIQEEKPKK KKKAQEDL
    • Predicted molecular weight

      59 kDa
    • Amino acids

      25 to 505
    • Tags

      His tag N-Terminus
    • Additional sequence information

      Full length mature protein minus the signal peptide.

Associated products

  • Related Products

    • Anti-ERp57 antibody (ab10287)
    • Anti-ERp57 antibody [MaP.Erp57] (ab13506)
    • Anti-ERp57 antibody (ab13507)
    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Anti-6X His tag® antibody [4D11] (ab5000)
    • Anti-ERp57 antibody (ab88098)

Specifications

Our Abpromise guarantee covers the use of ab92937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

General Info

  • Alternative names

    • 58 kDa glucose regulated protein
    • 58 kDa glucose-regulated protein
    • 58 kDa microsomal protein
    • Disulfide isomerase ER 60
    • Disulfide isomerase ER-60
    • Endoplasmic reticulum resident protein 57
    • Endoplasmic reticulum resident protein 60
    • ER p57
    • ER protein 57
    • ER protein 60
    • ERp 57
    • ERp57
    • ERp60
    • ERp61
    • Glucose Regulated Protein 58 Kd
    • GRP 57
    • GRP 58
    • GRP57
    • HsT17083
    • p58
    • PDIA 3
    • PDIA3
    • PDIA3_HUMAN
    • Phospholipase C alpha
    • PI PLC
    • Protein disulfide isomerase A3
    • Protein disulfide isomerase family A member 3
    • Protein disulfide-isomerase A3
    see all
  • Tissue specificity

    Detected in the flagellum and head region of spermatozoa (at protein level).
  • Sequence similarities

    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P30101 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant Human ERp57 protein (ab92937)
    SDS-PAGE - Recombinant Human ERp57 protein (ab92937)
    15% SDS-PAGE showing ab92937 (3µg) at approximately 58.5kDa.
  • Western blot - Recombinant Human ERp57 protein (ab92937)
    Western blot - Recombinant Human ERp57 protein (ab92937)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab92937? Please let us know so that we can cite the reference in this datasheet.

    ab92937 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    ERp57 functional assay

    Excellent
    Abreviews
    Abreviews
    Application
    Functional Studies
    We ordered the recombinant ERp57 for use in an experiment to examine the catalytic activity of the enzyme. Using the product provided by abcam, we were able to successful observe the catalytic reduction of insulin. Thus in our experience, this product can be used for functional studies.

    Abcam user community

    Verified customer

    Submitted Apr 07 2014

    Question

    ich hab folgendes Protein von Ihnen bestellt erp57 (ab92937).
    Können sie mir sagen, ob dieses noch biologisch aktiv ist??
    Vielen Dank im Vorraus

    Read More

    Abcam community

    Verified customer

    Asked on Oct 22 2012

    Answer

    Vielen Dank für Ihre Anfrage.

    Leider haben wir die Aktivität dieses Produktes nicht getestet.

    Da es allerdings mit 10% Glycerin und DTT versetzt wurde, gehe ich eher davon aus, dass dieses Protein nicht mehr aktiv ist.

    Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Oct 22 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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