Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: MS, Functional Studies, SDS-PAGE
Product nameRecombinant human ERp72 protein
Specific activity > 10 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.
Purity> 90 % SDS-PAGE.
ab206794 was expressed in E.coli and purified by using conventional chromatography techniques.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMGSSHHHHHHSSGLVPRGSHMVAGAEGPDEDSSNRENAIEDEEEEEEEDD DEEEDDLEVKEENGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAP EYEKIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILKKGQAV DYEGSRTQEEIVAKVREVSQPDWTPPPEVTLVLTKENFDEVVNDADIILV EFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATAETDLAKRFDV SGYPTLKIFRKGRPYDYNGPREKYGIVDYMIEQSGPPSKEILTLKQVQEF LKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLK VSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGH RKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYT FAIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLR EFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIE FYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEG FPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRTKEEL
Predicted molecular weight73 kDa including tags
Amino acids21 to 645
TagsHis tag N-Terminus
Additional sequence informationNP_004902. Mature protein without signal peptide.
Our Abpromise guarantee covers the use of ab206794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.32% Tris HCl, 0.58% Sodium chloride, 0.02% DTT, 10% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Calcium binding protein intestinal related
- Endoplasmic reticulum resident protein 70
- Endoplasmic reticulum resident protein 72
Sequence similaritiesBelongs to the protein disulfide isomerase family.
Contains 3 thioredoxin domains.
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab206794 has not yet been referenced specifically in any publications.