Recombinant Human EYA2 protein (ab124581)
- Datasheet
- References
- Protocols
Overview
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Product name
Recombinant Human EYA2 protein -
Protein length
Protein fragment
Description
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Nature
Recombinant -
Source
Escherichia coli -
Amino Acid Sequence
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Accession
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Species
Human -
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMERVFVW DLDETIIIFH SLLTGTFASR YGKDTTTSVR IGLMMEEMIF NLADTHLFFN DLEDCDQIHV DDVSSDDNGQ DLSTYNFSAD GFHSSAPAAN LCLGSGVHGG VDWMRKLAFR YRRVKEMYNT YKNNVGGLIG TPKRETWLQL RAELEALTDL WLTHSLKALN LINSRPNCVN VLVTTTQLIP ALAKVLLYGL GSVFPIENIY SATKTGKESC FERIMQRFGR KAVYVVIGDG VEEEQGAKKH NMPFWRISCH ADLEALRHAL ELEYL -
Molecular weight
33 kDa including tags -
Amino acids
244 to 514 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab124581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Purity
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 0.02% DTT, 0.32% Tris HCl, 40% Glycerol, 0.88% Sodium chloride
General Info
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Alternative names
- EAB 1
- EAB1
- EYA 2
see all -
Function
Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1. Seems to coactivate SIX2, SIX4 and SIX5. Together with SIX1 and DACH2 seem to be involved in myogenesis. May be involved in development of the eye. Interaction with GNAZ and GNAI2 prevents nuclear translocation and transcriptional activity. -
Tissue specificity
Highest expression in muscle with lower levels in kidney, placenta, pancreas, brain and heart. -
Sequence similarities
Belongs to the HAD-like hydrolase superfamily. EYA family. -
Developmental stage
At the begin of fourth week of development detected in cytoplasm of somite cells. Between the sixth and eighth week of development detected in cytoplasm of limb bud cells. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human EYA2 protein (ab124581)15% SDS-PAGE showing ab124581 (3µg).
Datasheets and documents
References
ab124581 has not yet been referenced specifically in any publications.
