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Signal Transduction Signaling Pathway G Protein Signaling Heterotrimeric G Proteins G Proteins
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Recombinant Human EYA2 protein (ab124581)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant Human EYA2 protein (ab124581)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant Human EYA2 protein
    • Purity

      > 90 % SDS-PAGE.
      ab123581 is purified using conventional chromatography techniques.
    • Expression system

      Escherichia coli
    • Accession

      O00167
    • Protein length

      Protein fragment
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSMERVFVW DLDETIIIFH SLLTGTFASR YGKDTTTSVR IGLMMEEMIF NLADTHLFFN DLEDCDQIHV DDVSSDDNGQ DLSTYNFSAD GFHSSAPAAN LCLGSGVHGG VDWMRKLAFR YRRVKEMYNT YKNNVGGLIG TPKRETWLQL RAELEALTDL WLTHSLKALN LINSRPNCVN VLVTTTQLIP ALAKVLLYGL GSVFPIENIY SATKTGKESC FERIMQRFGR KAVYVVIGDG VEEEQGAKKH NMPFWRISCH ADLEALRHAL ELEYL
      • Predicted molecular weight

        33 kDa including tags
      • Amino acids

        244 to 514
      • Tags

        His tag N-Terminus

    Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)

    Specifications

    Our Abpromise guarantee covers the use of ab124581 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF
    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.02% DTT, 0.32% Tris HCl, 40% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    General Info

    • Alternative names

      • EAB 1
      • EAB1
      • EYA 2
      • EYA transcriptional coactivator and phosphatase 2
      • EYA2
      • EYA2_HUMAN
      • eyes absent 2 homolog (Drosophila)
      • Eyes absent homolog 2
      • Eyes absent homolog 2 (Drosophila)
      • Translation of this uORF probably lowers the translation efficiency of EYA2
      see all
    • Function

      Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1. Seems to coactivate SIX2, SIX4 and SIX5. Together with SIX1 and DACH2 seem to be involved in myogenesis. May be involved in development of the eye. Interaction with GNAZ and GNAI2 prevents nuclear translocation and transcriptional activity.
    • Tissue specificity

      Highest expression in muscle with lower levels in kidney, placenta, pancreas, brain and heart.
    • Sequence similarities

      Belongs to the HAD-like hydrolase superfamily. EYA family.
    • Developmental stage

      At the begin of fourth week of development detected in cytoplasm of somite cells. Between the sixth and eighth week of development detected in cytoplasm of limb bud cells.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Target information above from: UniProt accession O00167 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human EYA2 protein (ab124581)
      SDS-PAGE - Recombinant Human EYA2 protein (ab124581)
      15% SDS-PAGE showing ab124581 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab124581? Please let us know so that we can cite the reference in this datasheet.

    ab124581 has not yet been referenced specifically in any publications.

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