Recombinant human FABP-1 protein (ab206788)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, MS, SDS-PAGE
Description
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Product name
Recombinant human FABP-1 protein
See all FABP-1 proteins and peptides -
Biological activity
Specific activity is > 60 units/mg, and is defined as the amount of enzyme that convert 1μmole of α-ketoglutarate to L-Glutamate per
minute at pH 8.0 at 25°C. -
Purity
> 95 % SDS-PAGE.
ab206788 was purified using conventional chromatography techniques. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSSSWWTHVEMGPPDPILGVTEAFKRDTN SKKMNLGVGAYRDDNGKPYVLPSVRKAEAQIAAKNLDKEYLPIGGLAEFC KASAELALGENSEVLKSGRFVTVQTISGTGALRIGASFLQRFFKFSRDVF LPKPTWGNHTPIFRDAGMQLQGYRYYDPKTCGFDFTGAVEDISKIPEQSV LLLHACAHNPTGVDPRPEQWKEIATVVKKRNLFAFFDMAYQGFASGDGDK DAWAVRHFIEQGINVCLCQSYAKNMGLYGERVGAFTMVCKDADEAKRVES QLKILIRPMYSNPPLNGARIAAAILNTPDLRKQWLQEVKVMADRIIGMRT QLVSNLKKEGSTHNWQHITDQIGMFCFTGLKPEQVERLIKEFSIYMTKDG RISVAGVTSSNVGYLAHAIHQVTK -
Predicted molecular weight
47 kDa including tags -
Amino acids
30 to 430 -
Tags
His tag N-Terminus -
Additional sequence information
This product is for the mature full length protein. The transit peptide is not included. NP_002071.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab206788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
Mass Spectrometry
SDS-PAGE
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Additional notes
Previously labelled as GOT2.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.87% Sodium chloride, 0.02% DTTThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- AATM_HUMAN
- AL022787
- Aspartate aminotransferase
see all -
Function
Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. -
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. -
Cellular localization
Mitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane. - Information by UniProt
Images
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Anti-FABP-1 antibody [EPR12354(B)] (ab171739) at 1/1000 dilution +
Recombinant human FABP-1 protein (ab206788) at 0.015 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 47 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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15% SDS-PAGE analysis of ab206788 (3μg).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab206788 has not yet been referenced specifically in any publications.