Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, MS, SDS-PAGE
Product nameRecombinant human FABP-1 protein
See all FABP-1 proteins and peptides
Specific activity is > 60 units/mg, and is defined as the amount of enzyme that convert 1μmole of α-ketoglutarate to L-Glutamate per
minute at pH 8.0 at 25°C.
Purity> 95 % SDS-PAGE.
ab206788 was purified using conventional chromatography techniques.
Endotoxin level< 1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMGSSHHHHHHSSGLVPRGSHMGSSSWWTHVEMGPPDPILGVTEAFKRDTN SKKMNLGVGAYRDDNGKPYVLPSVRKAEAQIAAKNLDKEYLPIGGLAEFC KASAELALGENSEVLKSGRFVTVQTISGTGALRIGASFLQRFFKFSRDVF LPKPTWGNHTPIFRDAGMQLQGYRYYDPKTCGFDFTGAVEDISKIPEQSV LLLHACAHNPTGVDPRPEQWKEIATVVKKRNLFAFFDMAYQGFASGDGDK DAWAVRHFIEQGINVCLCQSYAKNMGLYGERVGAFTMVCKDADEAKRVES QLKILIRPMYSNPPLNGARIAAAILNTPDLRKQWLQEVKVMADRIIGMRT QLVSNLKKEGSTHNWQHITDQIGMFCFTGLKPEQVERLIKEFSIYMTKDG RISVAGVTSSNVGYLAHAIHQVTK
Predicted molecular weight47 kDa including tags
Amino acids30 to 430
TagsHis tag N-Terminus
Additional sequence informationThis product is for the mature full length protein. The transit peptide is not included. NP_002071.
Our Abpromise guarantee covers the use of ab206788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Previously labelled as GOT2.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.87% Sodium chloride, 0.02% DTT
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Aspartate aminotransferase
FunctionPlays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.
Sequence similaritiesBelongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Cellular localizationMitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane.
- Information by UniProt
Anti-FABP-1 antibody [EPR12354(B)] (ab171739) at 1/1000 dilution +
Recombinant human FABP-1 protein (ab206788) at 0.015 µg with 5% NFDM/TBST
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 47 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
15% SDS-PAGE analysis of ab206788 (3μg).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab206788 has not yet been referenced specifically in any publications.