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Recombinant human FAK protein (ab105909)

  • Datasheet
Submit a review Submit a question References (1)

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Functional Studies - Recombinant human FAK protein (ab105909)
  • SDS-PAGE - Recombinant human FAK protein (ab105909)
  • SDS-PAGE - Recombinant human FAK protein (ab105909)
  • Functional Studies - Recombinant human FAK protein (ab105909)
  • SDS-PAGE - Recombinant human FAK protein (ab105909)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 85% SDS-PAGE
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

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Description

  • Product name

    Recombinant human FAK protein
    See all FAK proteins and peptides
  • Biological activity

    The Specific activity of ab105909 was determined to be 28 nmol/min/mg.
  • Purity

    > 85 % SDS-PAGE.
    Purity was determined to be >85% by densitometry.
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    Q05397
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      140 kDa
    • Amino acids

      1 to 1052
    • Tags

      GST tag N-Terminus

Associated products

  • Related Products

    • Anti-FAK antibody [EP695Y] (ab40794)
    • Anti-FAK (phospho S732) antibody (ab4792)
    • Anti-FAK antibody [63D5] (ab72140)
    • Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244)
    • Anti-FAK antibody [EP1831Y] (ab76496)
  • Substrate reagent

    • Poly (Glu, Tyr) peptide (ab204877)

Specifications

Our Abpromise guarantee covers the use of ab105909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.307% Glutathione, 0.01% Triton-X-100, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • FADK
    • FADK 1
    • FAK related non kinase polypeptide
    • FAK1
    • FAK1_HUMAN
    • Focal adhesion Kinase
    • Focal adhesion kinase 1
    • Focal adhesion kinase isoform FAK Del33
    • Focal adhesion kinase related nonkinase
    • FRNK
    • p125FAK
    • pp125FAK
    • PPP1R71
    • Protein phosphatase 1 regulatory subunit 71
    • Protein tyrosine kinase 2
    • Protein-tyrosine kinase 2
    • Ptk2
    • PTK2 protein tyrosine kinase 2
    see all
  • Function

    Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
  • Tissue specificity

    Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • Domain

    The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
  • Post-translational
    modifications

    Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
  • Cellular localization

    Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Target information above from: UniProt accession Q05397 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant human FAK protein (ab105909)
    Functional Studies - Recombinant human FAK protein (ab105909)
    The specific activity of FAK (ab105909) was determined to be 24 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human FAK protein (ab105909)
    SDS-PAGE - Recombinant human FAK protein (ab105909)
    SDS PAGE analysis of ab105909
  • SDS-PAGE - Recombinant human FAK protein (ab105909)
    SDS-PAGE - Recombinant human FAK protein (ab105909)
    SDS PAGE analysis of ab105909
  • Functional Studies - Recombinant human FAK protein (ab105909)
    Functional Studies - Recombinant human FAK protein (ab105909)
    Kinase Assay demonstrating specific activity of ab105909.
  • SDS-PAGE - Recombinant human FAK protein (ab105909)
    SDS-PAGE - Recombinant human FAK protein (ab105909)
    SDS-PAGE showing ab105909 at approximately 140kDa.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab105909? Please let us know so that we can cite the reference in this datasheet.

    ab105909 has been referenced in 1 publication.

    • Heim JB  et al. Myosin-1E interacts with FAK proline-rich region 1 to induce fibronectin-type matrix. Proc Natl Acad Sci U S A 114:3933-3938 (2017). Other ; Mouse, Human . PubMed: 28348210

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