Recombinant human FAK protein (ab105909)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameRecombinant human FAK protein
See all FAK proteins and peptides -
Protein lengthFull length protein
Description
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NatureRecombinant
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SourceBaculovirus infected Sf9 cells
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Amino Acid Sequence
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Accession
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SpeciesHuman
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Molecular weight140 kDa
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Amino acids1 to 1052
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TagsGST tag N-Terminus
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Associated products
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Affibody® Molecule
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ELISA pair antibody
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab105909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Biological activityThe Specific activity of ab105909 was determined to be 28 nmol/min/mg.
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Applications
Functional Studies
SDS-PAGE
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Purity> 85 % SDS-PAGE.
Purity was determined to be >85% by densitometry. -
FormLiquid
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Additional notes
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.01% Triton-X-100, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- FADK
- FADK 1
- FAK related non kinase polypeptide
see all -
FunctionNon-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
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Tissue specificityExpressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
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Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain. -
DomainThe first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions. -
Post-translational
modificationsPhosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1. -
Cellular localizationCell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
- Information by UniProt
Images
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Functional Studies - Recombinant human FAK protein (ab105909)Kinase Assay demonstrating specific activity of ab105909. -
SDS-PAGE - Recombinant human FAK protein (ab105909)SDS-PAGE showing ab105909 at approximately 140kDa.
Datasheets and documents
References
This product has been referenced in:
- Heim JB et al. Myosin-1E interacts with FAK proline-rich region 1 to induce fibronectin-type matrix. Proc Natl Acad Sci U S A 114:3933-3938 (2017). Other ; Mouse, Human . Read more (PubMed: 28348210) »
