Recombinant human FAK protein (ab105909)
- Datasheet
- References (1)
- Protocols
Description
-
Product name
Recombinant human FAK protein
See all FAK proteins and peptides -
Biological activity
The Specific activity of ab105909 was determined to be 28 nmol/min/mg. -
Purity
> 85 % SDS-PAGE.
Purity was determined to be >85% by densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Predicted molecular weight
140 kDa -
Amino acids
1 to 1052 -
Tags
GST tag N-Terminus
-
Associated products
-
Related Products
-
Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab105909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Functional Studies
SDS-PAGE
-
Form
Liquid -
Additional notes
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.01% Triton-X-100, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- FADK
- FADK 1
- FAK related non kinase polypeptide
see all -
Function
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity. -
Tissue specificity
Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain. -
Domain
The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions. -
Post-translational
modificationsPhosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1. -
Cellular localization
Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions. - Information by UniProt
Images
-
Functional Studies - Recombinant human FAK protein (ab105909)The specific activity of FAK (ab105909) was determined to be 24 nmol/min/mg as per activity assay protocol -
SDS-PAGE - Recombinant human FAK protein (ab105909)SDS PAGE analysis of ab105909 -
SDS-PAGE - Recombinant human FAK protein (ab105909)SDS PAGE analysis of ab105909 -
Functional Studies - Recombinant human FAK protein (ab105909)Kinase Assay demonstrating specific activity of ab105909. -
SDS-PAGE - Recombinant human FAK protein (ab105909)SDS-PAGE showing ab105909 at approximately 140kDa.
Datasheets and documents
References
This product has been referenced in:
- Heim JB et al. Myosin-1E interacts with FAK proline-rich region 1 to induce fibronectin-type matrix. Proc Natl Acad Sci U S A 114:3933-3938 (2017). Other ; Mouse, Human . Read more (PubMed: 28348210) »
