Recombinant Human FANCI protein (ab132153)
Key features and details
- Expression system: Wheat germ
- Purity: >= 80% Purified via GST Tag
- Tags: GST tag N-Terminus
- Suitable for: ELISA, SDS-PAGE, WB
Description
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Product name
Recombinant Human FANCI protein -
Purity
>= 80 % Purified via GST Tag.
Glutathione Sepharose -
Expression system
Wheat germ -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MFKDVPLTAEEVEFVVEKALSMFSKMNLQEIPPLVYQLLVLSSKGSRKSV LEGIIAFFSALDKQHNEEQSGDE -
Predicted molecular weight
34 kDa including tags -
Amino acids
180 to 252 -
Tags
GST tag N-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab132153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
SDS-PAGE
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
General Info
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Alternative names
- FANCI
- FANCI gene
- FANCI_HUMAN
see all -
Function
Required for maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. Participates in S phase and G2 phase checkpoint activation upon DNA damage. Promotes FANCD2 ubiquitination and recruitment to DNA repair sites. -
Involvement in disease
Defects in FANCI are a cause of Fanconi anemia complementation group I (FANCI) [MIM:609053]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. -
Domain
The C-terminal 30 residues are probably required for function in DNA repair. -
Post-translational
modificationsMonoubiquitinated on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression.
Phosphorylated in response to DNA damage by ATM and/or ATR. -
Cellular localization
Nucleus. Concentrates in nuclear foci upon genotoxic stress. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab132153 has not yet been referenced specifically in any publications.