The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ED50: 50ng/ml (A20 cells). Kills Jurkat cells at concentrations of >10ng/ml in the absence of a cross-linker. Cross-linking enhancer increases the activity of rhsFasL approx. 50-fold.
Please note: Results using sFasL may differ from those obtained with agonistic antibodies.
> 0.100 Eu/µg
>95% by SDS-PAGE . ab157085 is purified by Multi-Step Chromatography.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: 99% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
Apoptosis (APO 1) antigen ligand 1
Apoptosis antigen ligand
Apoptosis antigen ligand 1
Fas antigen ligand
Fas ligand (TNF superfamily member 6)
Fasl Fas ligand (TNF superfamily member 6)
Generalized lymphoproliferative disease
Tumor necrosis factor (ligand) superfamily member 6
Tumor necrosis factor ligand superfamily member 6
Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.
Involvement in disease
Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
Belongs to the tumor necrosis factor family.
N-glycosylated. The soluble form derives from the membrane form by proteolytic processing.
Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface.
Functional Studies - Fas Ligand protein (Active) (ab157085)
Activity Assay analysis: Inhibition of ab157085-mediated lysis. Fas (human):Fc (Human) (rec.) exerts its inhibitory activity in a concentration range of 0.5-5µg/ml in the presence of the enhancer (1µg/ml).
has not yet been referenced specifically in any publications.
Publishing research using ab157085? Please let us know so that we can cite the reference in this datasheet.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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