Recombinant human FBP2 protein (ab208316)
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- References
- Protocols
Description
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Product name
Recombinant human FBP2 protein
See all FBP2 proteins and peptides -
Biological activity
Specific activity is > 1,500 pmol/min/ug obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 pmole of fructose 1,6 diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 37C.
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Purity
> 90 % SDS-PAGE.
ab208316 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSMTDRSPFETDMLTLTRYVMEKGRQAKG TGELTQLLNSMLTAIKAISSAVRKAGLAHLYGIAGSVNVTGDEVKKLDVL SNSLVINMVQSSYSTCVLVSEENKDAIITAKEKRGKYVVCFDPLDGSSNI DCLASIGTIFAIYRKTSEDEPSEKDALQCGRNIVAAGYALYGSATLVALS TGQGVDLFMLDPALGEFVLVEKDVKIKKKGKIYSLNEGYAKYFDAATTEY VQKKKFPEDGSAPYGARYVGSMVADVHRTLVYGGIFLYPANQKSPKGKLR LLYECNPVAYIIEQAGGLATTGTQPVLDVKPEAIHQRVPLILGSPEDVQE YLTCVQKNQAGS -
Predicted molecular weight
39 kDa including tags -
Amino acids
1 to 339 -
Tags
His tag N-Terminus -
Additional sequence information
NP_003828.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab208316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8
Constituents: 0.32% Tris HCl, 0.87% Sodium chloride, 10% Glycerol, 0.02% DTTThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 6-bisphosphatase isozyme 2
- 6-bisphosphate 1-phosphohydrolase 2
- D fructose 1 6 bisphosphate 1 phosphohydrolase 2
see all -
Function
Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations and probably participates in glycogen synthesis from carbohydrate precursors, such as lactate. -
Tissue specificity
Expressed in skeletal muscle (at protein level). -
Pathway
Carbohydrate biosynthesis; gluconeogenesis. -
Sequence similarities
Belongs to the FBPase class 1 family. -
Cellular localization
Cell junction. Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, Z line. In neonatal cardiomyocytes, distributed throughout the cytosol, accumulating in the intercalated disks which occur at the Z line of cardiomyocytes and connect adjacent cells, and also located in the nucleus; dissociates from the Z line following an increase in cytosolic Ca(2+) concentration (By similarity). In muscle precursor cells, localizes predominantly to the nucleus and to a lesser extent to the cytoplasm at the proliferative phase, while mainly localizing to the cytoplasm at the differentiation phase (By similarity). Colocalizes with ALDOA and alpha-actinin on both sides of the Z line of skeletal muscle; dissociates rapidly from the Z line following an increase in cytosolic Ca(2+) concentration. - Information by UniProt
Images
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SDS-PAGE - Recombinant human FBP2 protein (ab208316)15% SDS-PAGE analysis of ab208316 (3µg).
Datasheets and documents
References
ab208316 has not yet been referenced specifically in any publications.
