Description

  • Product name

    Recombinant Human FEN1 protein
  • Purity

    > 90 % SDS-PAGE.
    ab95382 is purified using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQAAGAE QEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALVKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RILQELGLNQ EQFVDLCILL GSDYCESIRG IGPKRAVDLI QKHKSIEEIV RRLDPNKYPV PENWLHKEAH QLFLEPEVLD PESVELKWSE PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGSTKKKAKT GAAGKFKRGK

Specifications

Our Abpromise guarantee covers the use of ab95382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

    Western blot

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol

General Info

  • Alternative names

    • DNase IV
    • FEN-1
    • FEN1
    • FEN1_HUMAN
    • Flap endonuclease 1
    • Flap structure specific endonuclease 1
    • Flap structure-specific endonuclease 1
    • hFEN-1
    • hFEN1
    • Maturation factor 1
    • MF1
    • Rad2
    see all
  • Function

    Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
  • Sequence similarities

    Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
  • Post-translational
    modifications

    Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
    Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
    Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.
  • Cellular localization

    Nucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of 3µg ab95382.

References

ab95382 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Fen1

Good
Abreviews
Application
Other
Coomassie staining

Abcam user community

Verified customer

Submitted Jan 24 2018

Question
Answer

Thank you for contacting us. The lab has confirmed that this protein is not tagged. The sequence of this protein is : MGIQGLAKLI ADVAPSAIR E NDIKSYFG K VAIDASMS Y QFLIAVRQ G DVLQNEEG T TSHLMGMF R TIRMMENG K PVYVFDGK P QLKSGELA R SERRAEAE Q LQQAQAAG E QEVEKFTK L VKVTKQHN E CKHLLSLM I PYLDAPSE E ASCAALVK G KVYAAATE M DCLTFGSP L MRHLTASE K KLPIQEFH S RILQELGL Q EQFVDLCI L GSDYCESI G IGPKRAVD I QKHKSIEE V RRLDPNKY V PENWLHKE H QLFLEPEV D PESVELKW E PNEEELIK M CGEKQFSE R IRSGVKRL K SRQGSTQG L DDFFKVTG L SSAKRKEP P KGSTKKK KT GAAGKFK GK I hope this helps, please let me know if you need any additional information.

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