Recombinant human FES protein (ab196084)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human FES protein
See all FES proteins and peptides -
Biological activity
Specific activity of 235 pmol/min/µg.
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Purity
> 90 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
GFSSELCSPQGHGVLQQMQEAELRLLEGMRKWMAQRVKSDREYAGLLHHM SLQDSGGQSRAISPDSPISQSWAEITSQTEGLSRLLRQHAEDLNSGPLSK LSLLIRERQQLRKTYSEQWQQLQQELTKTHSQDIEKLKSQYRALARDSAQ AKRKYQEASKDKDRDKAKDKYVRSLWKLFAHHNRYVLGVRAAQLHHQHHH QLLLPGLLRSLQDLHEEMACILKEILQEYLEISSLVQDEVVAIHREMAAA AARIQPEAEYQGFLRQYGSAPDVPPCVTFDESLLEEGEPLEPGELQLNE LTVESVQHTLTSVTDELAVATEMVFRRQEMVTQLQQELRNEEENTHPRE RVQLLGKRQVLQEALQGLQVALCSQAKLQAQQELLQTKLEHLGPGEPPPV LLLQDDRHSTSSSEQEREGGR TPTLEILKSHISGIFRPKFSLPPPLQL IPEVQKPLHEQLWYHGAIPRAEVAELLVHSGDFLVRESQGKQEYVLSVLW DGLPRHFIIQSLDNLYRLEGEGFPSIPLLIDHLLSTQQPLTKKSGVVLHR AVPKDKWVLNHEDLVLGEQIGRGNFGEVFSGRLRADNTLVAVKSCRETLP PDLKAKFLQEARILKQYSHPNIVRLIGVCTQKQPIYIVMELVQGGDFLTF LRTEGARLRVKTLLQMVGDAAAGMEYLESKCCIHRDLAARNCLVTEKNVL KISDFGMSREEADGVYAASGGLRQVPVKWTAPEALNYGRYSSESDVWSFG ILLWETFSLGASPYPNLSNQQTREFVEKGGRLPCPELCPDAVFRLMEQCW AYEPGQRPSFSTIYQELQSIRKRHR -
Predicted molecular weight
95 kDa including tags -
Amino acids
2 to 822 -
Tags
His-DDDDK tag N-Terminus -
Additional sequence information
Genbank #: NM_002005
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Associated products
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Related Products
- Anti-FES antibody [EPR3230(2)] (ab108418)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-FES antibody (ab153841)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab21536)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-6X His tag® antibody (ab9108)
Specifications
Our Abpromise guarantee covers the use of ab196084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.64% Tris HCl, 0.64% Sodium chloride, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.017% Potassium chloride, 20% Glycerol (glycerin, glycerine)
Buffer also contains FLAG peptide.This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- c-fes/fps protein
- cFES
- Feline sarcoma (Snyder Theilen) viral (v fes)/Fujinami avian sarcoma (PRCII) viral (v fps) oncogene homolog
see all -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
Contains 1 FCH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain. - Information by UniProt
Images
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Analysis of ATP transfer with various amounts of ab196084. Assay was performed in a Kinase buffer (40 mM Tris pH 7.4, 10 mM MgCl2, 0.1 mg/mL BSA) containing 1 mM DTT using Poly-(Glu4:Tyr)substrate (0.2 mg/mL) and 20 µM ATP for 30 minutes at 30°C.
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10% SDS-PAGE analysis of ab196084 stained with Coomassie Blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab196084 has not yet been referenced specifically in any publications.