Recombinant human FGF 23 protein (ab108553)



  • Nature
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
    • Molecular weight
      60 kDa including tags
    • Amino acids
      1 to 251

Associated products


Our Abpromise guarantee covers the use of ab108553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Activates ERK and FRS2alpha phosphorylation in Klotho expressing cells.
  • Applications

    Functional Studies


  • Endotoxin level
    < 0.100 Eu/µg
  • Purity
    > 90 % SDS-PAGE.
    ab108553 is 0.2µm filtered.
  • Form
  • Additional notes
    Working aliquots are stable for up to 3 months when stored at -20°C.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • ADHR
    • FGF-23
    • Fgf23
    • FGF23_HUMAN
    • FGFN
    • Fibroblast growth factor 23
    • Fibroblast growth factor 23 C-terminal peptide
    • Fibroblast growth factor 23 precursor
    • HPDR2
    • HYPF
    • Phosphatonin
    • PHPTC
    • Tumor derived hypophosphatemia inducing factor
    • Tumor-derived hypophosphatemia-inducing factor
    see all
  • Function
    Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization.
  • Tissue specificity
    Expressed in osteogenic cells particularly during phases of active bone remodeling. In adult trabecular bone, expressed in osteocytes and flattened bone-lining cells (inactive osteoblasts).
  • Involvement in disease
    Defects in FGF23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR) [MIM:193100]. ADHR is characterized by low serum phosphorus concentrations, rickets, osteomalacia, leg deformities, short stature, bone pain and dental abscesses.
    Defects in FGF23 are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. HFTC is a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues.
  • Sequence similarities
    Belongs to the heparin-binding growth factors family.
  • Post-translational
    Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases.
    O-glycosylated by GALT3. Glycosylation is necessary for secretion; it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23.
  • Cellular localization
    Secreted. Secretion is dependent on O-glycosylation.
  • Information by UniProt


  • ERK and FRS2aplha phosphorylation induced by FGF 23 in Klotho expressing cells.
    Klotho expressing HEK 293EBNA cells were serum starved for 16hr and then stimulated with hFGF 23-His, FGF 23-Fc (ab108553), mCD137-Fc (Fc control) and FGF-b (positive control) for 10 min, respectively.
    Antibodies against pFRS2alpha, pERK1/2 & total ERK1/2 were used for immunoblotting.

    Lane 1: Mock (non-treated)
    Lane 2: Mock + hFGF 23-Fc (ab108553) 1µg/ml
    Lane 3: Mock + hFGF 23-His 1µg/ml
    Lane 4: Klotho + hFGF 23-Fc (ab108553) 1µg/ml
    Lane 5: Klotho + hFGF 23-Fc (ab108553) 4µg/ml
    Lane 6: Klotho + mCD137-Fc 1µg/ml
    Lane 7: Klotho (non-treated)
    Lane 8: Klotho + 100ng/ml FGF-b


ab108553 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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