Key features and details
- Expression system: Escherichia coli
- Purity: >= 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human FGF1 protein (Active)
See all FGF1 proteins and peptides
The ED50 of ab268551 was determined by the dose-dependent stimulation of mouse fibroblasts and it was <0.5ng/ml.
Purity>= 95 % SDS-PAGE.
Endotoxin level< 1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthProtein fragment
Molecular weight informationSDS-PAGE molecular weight: ~16kDa
Additional sequence informationMature chain.
Our Abpromise guarantee covers the use of ab268551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Buffer lyophilised from.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionA quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
- Acidic fibroblast growth factor
- Beta endothelial cell growth factor
FunctionThe heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
Sequence similaritiesBelongs to the heparin-binding growth factors family.
Cellular localizationSecreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268551 has not yet been referenced specifically in any publications.