Bioactive grade

Recombinant human FGF10 protein (Animal Free) (ab218085)

Description

  • Product name

    Recombinant human FGF10 protein (Animal Free)
    See all FGF10 proteins and peptides
  • Biological activity

    The activity is determined by a proliferation assay using 4MBr-5 rhesus monkey epithelial cells and is typically 20-100 ng/ml. This corresponds to an expected specific activity of 5 x 104 units/mg.

  • Purity

    = 95 % SDS-PAGE.
    The purity of ab218085 is determined by Reducing and Non-reducing SDS-PAGE.
  • Endotoxin level

    < 0.050 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    Yes
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MLGQDMVSPEATNSSSSSFSSPSSAGRHVRSYNHLQGDVRWRKLFSFTKY FLKIEKNGKVSGTKKENCPYSILEITSVEIGVVAVKAINSNYYLAMNKKG KLYGSKEFNNDCKLKERIEENGYNTYASFNWQHNGRQMYVALNGKGAPRR GQKTRRKNTSAHFLPMVVHS
    • Predicted molecular weight

      19 kDa
    • Amino acids

      40 to 208
    • Additional sequence information

      Non-glycosylated protein.

Specifications

Our Abpromise guarantee covers the use of ab218085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituent: 0.16% Sodium phosphate

    Lyophilised from a sterile filtered solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute in sterile water at 0.1 mg/ml. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 ºC and avoid repeat freeze thaws.

General Info

  • Alternative names

    • BB213776
    • fd11d03
    • FGF 10
    • FGF-10
    • FGF10
    • FGF10_HUMAN
    • Fibroblast growth factor 10
    • Keratinocyte growth factor 2
    • KGF 2
    • Produced by fibroblasts of urinary bladder lamina propria
    • wu:fd11d03
    • zgc:109774
    see all
  • Function

    Could be a growth factor active in the process of wound healing. Acts as a mitogen in the lung. May act in a manner similar to FGF-7.
  • Involvement in disease

    Defects in FGF10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands (ALSG) [MIM:180920]. ALSG has variable expressivity, and affected individuals may have aplasia or hypoplasia of the lacrimal, parotid, submandibular and sublingual glands and absence of the lacrimal puncta. The disorder is characterized by irritable eyes, recurrent eye infections, epiphora (constant tearing) and xerostomia (dryness of the mouth), which increases the risk of dental erosion, dental caries, periodontal disease and oral infections.
    Defects in FGF10 are a cause of lacrimo-auriculo-dento-digital syndrome (LADDS) [MIM:149730]; also known as Levy-Hollister syndrome. LADDS is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. LADDS is an autosomal dominant syndrome characterized by aplastic/hypoplastic lacrimal and salivary glands and ducts, cup-shaped ears, hearing loss, hypodontia and enamel hypoplasia, and distal limb segments anomalies. In addition to these cardinal features, facial dysmorphism, malformations of the kidney and respiratory system and abnormal genitalia have been reported. Craniosynostosis and severe syndactyly are not observed.
  • Sequence similarities

    Belongs to the heparin-binding growth factors family.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • Functional analysis of ab218085
  •  SDS PAGE analysis of ab218085 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.

  • Induced proliferation of 4MBr‐5 rhesus monkey epithelial cells using ab218085.

References

ab218085 has not yet been referenced specifically in any publications.

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