The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Determined by dose-dependent ability to reduce tetrazolium salt, WST-8, by dehydrogenase activities of BaF3 cells expressing FGF receptors using Cell Counting Kit-8 (CCK-8).
Human FGF-16 is fully biologically active when compared to standards.
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is < 0.5 ng/ml, corresponding to a specific activity of > 2 x 106 units/mg
For most in vitro applications, FGF-16 exerts its biological activity in the concentration range of 0.1 to 20.0 ng/ml.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Please reconsititue this product in 50ul water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Fibroblast growth factor 16
Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation, and is required for normal cardiomyocyte proliferation and heart development.
Belongs to the heparin-binding growth factors family.