Bioactive grade

Recombinant human FGF2 protein (Active) (ab9596)

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Description

  • Product name

    Recombinant human FGF2 protein (Active)
    See all FGF2 proteins and peptides

  • Biological activity

    Determined by a cell proliferation assay using Balb/c 3T3 cells.

    The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

  • Purity

    >= 95 % SDS-PAGE.
    >= 95% HPLC Sterile filtered

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    Escherichia coli

  • Accession

    P09038

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      AAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS

    • Predicted molecular weight

      17 kDa

    • Amino acids

      135 to 288

Specifications

Our Abpromise guarantee covers the use of ab9596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Cell differentiation

    SDS-PAGE

    Cellular Activation

    HPLC

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.60
    Constituents: 0.0605% Tris, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Please reconstitute this product in 500ul 5mM Tris, pH7.6.

General Info

  • Alternative names

    • Basic fibroblast growth factor
    • Basic fibroblast growth factor bFGF
    • BFGF
    • FGF 2
    • FGF B
    • FGF-2
    • Fgf2
    • FGF2 basic
    • FGF2_HUMAN
    • FGFB
    • Fibroblast growth factor 2
    • Fibroblast growth factor 2 (basic)
    • Fibroblast growth factor, basic
    • HBGF 2
    • HBGF-2
    • HBGF2
    • HBGH 2
    • HBGH2
    • Heparin binding growth factor 2 precursor
    • Heparin-binding growth factor 2
    • Prostatropin
    see all

  • Function

    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).

  • Tissue specificity

    Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.

  • Sequence similarities

    Belongs to the heparin-binding growth factors family.

  • Post-translational
    modifications

    Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.

  • Cellular localization

    Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt

Images

  • Sandwich ELISA - Recombinant human FGF2 protein (ab9596)
    Sandwich ELISA - Recombinant human FGF2 protein (ab9596)
    Standard Curve for FGF basic (Analyte: FGF basic protein (Active) (ab9596)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS24] to FGF basic (ab17279) at 1ug/ml and Detector Antibody Rabbit monoclonal [EP1735] to FGF basic (ab92337) at 0.5ug/ml.
  • Cell differentiation - Recombinant human FGF2 protein (ab9596)
    Cell differentiation - Recombinant human FGF2 protein (ab9596)This image is courtesy of Sun Yung, Centro de Investigación Príncipe Felipe.
    Human embryonic stem cells (H9) were differentiated both as embryoid bodies and monolayer cultures (on Matrigel) using a protocol described in Kennedy et al., 2007. BMP-4, FGF-basic (ab9596) and VEGF (ab9571) were sequentially added to the media to direct differentiation. These images were taken at day 8, where cell adhesion antigens CD31 (ab32457 in green) and CD34 (ab8536 in red) are abundant and represent primitive haematopoietic cells within the culture. Dapi staining is shown in blue.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab9596? Please let us know so that we can cite the reference in this datasheet.

ab9596 has been referenced in 1 publication.

  • Dias MH  et al. Fibroblast Growth Factor 2 lethally sensitizes cancer cells to stress-targeted therapeutic inhibitors. Mol Oncol 13:290-306 (2019). PubMed: 30422399

Customer reviews and Q&As

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Question

Ab9863: BATCH NUMBER 107563 ORDER NUMBER 79846 DESCRIPTION OF THE PROBLEM This recombinant protein is lyophilized. For our applications the buffer salt concentration is critical. We need to know the volume of the product at the time of lyophilization. Ab9587: BATCH NUMBER 107562 ORDER NUMBER 79846 DESCRIPTION OF THE PROBLEM This recombinant protein is lyophilized. For our applications the buffer salt concentration is critical. We need to know the volume of the product at the time of lyophilization. Ab9596: BATCH NUMBER 107561 ORDER NUMBER 79846 DESCRIPTION OF THE PROBLEM This recombinant protein is lyophilized. For our applications the buffer salt concentration is critical. We need to know the volume of the product at the time of lyophilization.

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Answer

Thank you for your enquiry and thank you very much for your patience. I do apologize for the delay, it some time to get this information from the originator of these peptides. For each of the products listed, included is the concentration of each product prior to vialing and the volume required for each sample size listed. Ab9863: 10mg/ml 50ul/vial = 500ug Ab9587: 1mg/ml 100ul/vial = 100ug Ab9596: 2mg/ml 25ul/vial = 50ug If you have any additional questions, please contact us again.

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Question

hi, does your recombinant human basic FGF activate rat receptors?

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Answer

We do not test our product for cross reactivity however, we have customer feed back. I have checked our chart and I can confirm there is a positive reaction with rat.

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