Bioactive grade

Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) (ab108556)

Description

  • Product name

    Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active)
  • Biological activity

    Activates ERK and FRS2alpha phosphorylation in bKlotho expressing cells.
  • Purity

    >= 95 % SDS-PAGE.
    ab108556 is 0.2 µm filtered.
  • Endotoxin level

    < 0.100 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      50 kDa including tags
    • Additional sequence information

      Human FGF21 (aa 1-209) is fused at the C-terminus to the Fc portion of human IgG1.
  • Description

    Recombinant human FGF21 + IgG1 protein (Fc Chimera Active)

Specifications

Our Abpromise guarantee covers the use of ab108556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    Working aliquots are stable for up to 3 months when stored at -20°C.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Constituent: PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Cellular localization

    FGF21: Secreted. IgG1: Secreted. Cell membrane; Single-pass membrane protein

Images

  • ERK and FRS2alpha phosphorylation induced by FGF21 in bKlotho expressing cells.
    bKlotho expressing cells were serum starved for 16hr and then stimulated with hFGF21-His (competitor), ab108556 (FGF 21-Fc), mCD137-Fc (Fc control) and FGF-b (positive control) for 10 minutes, respectively.
    Antibodies against pFRS2alpha, pERK1/2 & total ERK1/2 were used for immunoblotting.

    Lane 1: 1 μg/ml hFGF21-His (competitor)
    Lane 2: 2.2 μg/ml ab108556 (hFGF21-Fc)
    Lane 3: 2.2 μg/ml mCD137-Fc
    Lane 4: 100 ng/μl FGF-b
    Lane 5: control (non-treated)

References

ab108556 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab108556.
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