The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Activates ERK and FRS2alpha phosphorylation in bKlotho expressing cells.
< 0.100 Eu/µg
% SDS-PAGE. ab108556 is 0.2 µm filtered.
Working aliquots are stable for up to 3 months when stored at -20°C.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
IgG1: Secreted. Cell membrane; Single-pass membrane protein
Functional Studies - Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) (ab108556)
ERK and FRS2alpha phosphorylation induced by FGF21 in bKlotho expressing cells.
bKlotho expressing cells were serum starved for 16hr and then stimulated with hFGF21-His (competitor), ab108556 (FGF
21-Fc), mCD137-Fc (Fc control) and FGF-b (positive control) for 10 minutes, respectively.
Antibodies against pFRS2alpha, pERK1/2 & total ERK1/2 were used for immunoblotting.
Lane 1: 1 μg/ml hFGF21-His (competitor)
Lane 2: 2.2 μg/ml ab108556 (hFGF21-Fc)
Lane 3: 2.2 μg/ml mCD137-Fc
Lane 4: 100 ng/μl FGF-b
Lane 5: control (non-treated)
has not yet been referenced specifically in any publications.
Publishing research using ab108556? Please let us know so that we can cite the reference in this datasheet.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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