Recombinant human FGF21 protein (Active) (ab54141)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, HPLC
Achieve higher consistency and quality standards with a premium grade bioactive protein
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
Description
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Product name
Recombinant human FGF21 protein (Active)
See all FGF21 proteins and peptides -
Biological activity
Determined by its ability to stimulate the proliferation of murine NIH-3T3 cells.
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Purity
> 90 % SDS-PAGE.
>90% HPLC analyses. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVK TSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPALPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS -
Predicted molecular weight
20 kDa -
Amino acids
29 to 209 -
Additional sequence information
Full length Mature protein, minus the signal peptide.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab54141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
HPLC
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Form
Lyophilized -
Additional notes
For extended storage, further dilute the reconstituted protein in a buffer (example PBS) containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstituted FGF-21 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. We recommend to reconstitute it in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For lot specific reconstitution information please contact our Scientific Support Team.
General Info
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Alternative names
- FGF 21
- FGF-21
- Fgf21
see all -
Function
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab54141 has been referenced in 1 publication.
- Blanco AM et al. FGF21 Mimics a Fasting-Induced Metabolic State and Increases Appetite in Zebrafish. Sci Rep 10:6993 (2020). PubMed: 32332781