Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, HPLC
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
Product nameRecombinant human FGF21 protein (Active)
See all FGF21 proteins and peptides
Determined by its ability to stimulate the proliferation of murine NIH-3T3 cells.
Purity> 90 % SDS-PAGE.
>90% HPLC analyses.
Endotoxin level< 1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVK TSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPALPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS
Predicted molecular weight20 kDa
Amino acids29 to 209
Additional sequence informationFull length Mature protein, minus the signal peptide.
Our Abpromise guarantee covers the use of ab54141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
For extended storage, further dilute the reconstituted protein in a buffer (example PBS) containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstituted FGF-21 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. We recommend to reconstitute it in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For lot specific reconstitution information please contact our Scientific Support Team.
- FGF 21
FunctionStimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.
Sequence similaritiesBelongs to the heparin-binding growth factors family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab54141 has been referenced in 1 publication.
- Blanco AM et al. FGF21 Mimics a Fasting-Induced Metabolic State and Increases Appetite in Zebrafish. Sci Rep 10:6993 (2020). PubMed: 32332781