Recombinant human FGF5 protein (Animal Free) (ab222181)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human FGF5 protein (Animal Free)
See all FGF5 proteins and peptides -
Biological activity
NR6R-3T3 proliferation with 1μg heparin.
ED50 < 10ng/mL.
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
<=1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
Yes -
Nature
Recombinant -
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Species
Human -
Sequence
MAWAHGEKRL APKGQPGPAA TDRNPIGSSS RQSSSSAMSS SSASSSPAAS LGSQGSGLEQ SSFQWSPSGR RTGSLYCRVG IGFHLQIYPD GKVNGSHEAN MLSVLEIFAV SQGIVGIRGV FSNKFLAMSK KGKLHASAKF TDDCKFRERF QENSYNTYAS AIHRTEKTGR EWYVALNKRG KAKRGCSPRV KPQHISTHFL PRFKQSEQPE LSFTVTVPEK KNPPSPIKSK IPLSAPRKNT NSVKYRLKFR FG -
Predicted molecular weight
28 kDa -
Amino acids
18 to 268
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Description
Recombinant human FGF5 protein
Specifications
Our Abpromise guarantee covers the use of ab222181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at -20°C. Avoid freeze / thaw cycle.
Constituents: 0.58% Sodium chloride, 0.16% Sodium phosphate
Lyophilization bufferThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute in sterile water at 0.1 mg/mL. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
General Info
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Alternative names
- FGF 5
- FGF-5
- FGF5
see all -
Function
Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase. -
Tissue specificity
Expressed in neonatal brain. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Developmental stage
Can transform NIH 3T3 cells. -
Cellular localization
Secreted. - Information by UniProt
Images
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SDS-PAGE analysis using ab222181 at 1μg under non-reducing (-) and reducing (+) conditions in a 4-20% Tris-Glycine gel, stained with Coomassie Blue.
Predicted MW: 27.7 kDa -
Human FGF5 induced proliferation of NR6R-3T3 cells.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab222181 has not yet been referenced specifically in any publications.