Bioactive grade

Recombinant human FGF5 protein (Animal Free) (ab222181)

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SDS-PAGE - Recombinant human FGF5 protein (Animal Free) (ab222181)
  • Functional Studies - Recombinant human FGF5 protein (Animal Free) (ab222181)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: <= 1.000 Eu/µg
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant human FGF5 protein (Animal Free)
    See all FGF5 proteins and peptides

  • Biological activity

    NR6R-3T3 proliferation with 1μg heparin.

    ED50 < 10ng/mL.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    <=1.000 Eu/µg

  • Expression system

    Escherichia coli

  • Accession

    P12034

  • Protein length

    Full length protein

  • Animal free

    Yes

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MAWAHGEKRL APKGQPGPAA TDRNPIGSSS RQSSSSAMSS SSASSSPAAS LGSQGSGLEQ SSFQWSPSGR RTGSLYCRVG IGFHLQIYPD GKVNGSHEAN MLSVLEIFAV SQGIVGIRGV FSNKFLAMSK KGKLHASAKF TDDCKFRERF QENSYNTYAS AIHRTEKTGR EWYVALNKRG KAKRGCSPRV KPQHISTHFL PRFKQSEQPE LSFTVTVPEK KNPPSPIKSK IPLSAPRKNT NSVKYRLKFR FG

    • Predicted molecular weight

      28 kDa

    • Amino acids

      18 to 268

  • Description

    Recombinant human FGF5 protein

Specifications

Our Abpromise guarantee covers the use of ab222181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at Room Temperature. Store at -20°C. Avoid freeze / thaw cycle.

    Constituents: 0.58% Sodium chloride, 0.16% Sodium phosphate

    Lyophilization buffer

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute in sterile water at 0.1 mg/mL. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

General Info

  • Alternative names

    • FGF 5
    • FGF-5
    • FGF5
    • FGF5_HUMAN
    • Fibroblast growth factor 5
    • HBGF 5
    • HBGF-5
    • heparin binding growth factor 5
    • Heparin-binding growth factor 5
    • Smag 82
    • Smag-82
    • TCMGLY
    see all

  • Function

    Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase.

  • Tissue specificity

    Expressed in neonatal brain.

  • Sequence similarities

    Belongs to the heparin-binding growth factors family.

  • Developmental stage

    Can transform NIH 3T3 cells.

  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human FGF5 protein (Animal Free) (ab222181)
    SDS-PAGE - Recombinant human FGF5 protein (Animal Free) (ab222181)

    SDS-PAGE analysis using ab222181 at 1μg under non-reducing (-) and reducing (+) conditions in a 4-20% Tris-Glycine gel, stained with Coomassie Blue. 
    Predicted MW: 27.7 kDa

  • Functional Studies - Recombinant human FGF5 protein (Animal Free) (ab222181)
    Functional Studies - Recombinant human FGF5 protein (Animal Free) (ab222181)

    Human FGF5 induced proliferation of NR6R-3T3 cells.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab222181? Please let us know so that we can cite the reference in this datasheet.

ab222181 has not yet been referenced specifically in any publications.

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