Key features and details
- Expression system: Escherichia coli
- Purity: >= 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human FGF8 protein (Active)
See all FGF8 proteins and peptides
The bioactivity of ab268560 was determined in a HIH/3T3 cell proliferation assay and the ED50 was in the range of 0.05-0.5 ng/ml.
Purity>= 95 % SDS-PAGE.
Endotoxin level< 1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthProtein fragment
Molecular weight informationSDS-PAGE molecular weight: ~22.5kDa
Our Abpromise guarantee covers the use of ab268560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.32% Sodium phosphate, 0.006% EDTA, 5.8% Sodium chloride
Buffer lyophilized from.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionA quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
- Androgen induced growth factor
- Androgen-induced growth factor
FunctionStimulates growth of the cells in an autocrine manner. Mediates hormonal action on the growth of cancer cells.
Involvement in diseaseDefects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6) [MIM:612702]. Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous.
Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
Sequence similaritiesBelongs to the heparin-binding growth factors family.
Developmental stageIn adults expression is restricted to the gonads.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268560 has not yet been referenced specifically in any publications.