Recombinant human FGF8 protein (Animal Free) (ab205519)
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Description
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Product name
Recombinant human FGF8 protein (Animal Free)
See all FGF8 proteins and peptides -
Biological activity
The activity is determined by its ability to induce proliferation of mouse 3T3 cells and is typically less than 20 ng/mL. This corresponds to an expected specific activity of 5 x 104 units/mg.
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Purity
> 97 % SDS-PAGE. -
Endotoxin level
< 0.050 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
Yes -
Nature
Recombinant -
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Species
Human -
Sequence
MQVTVQSSPNFTQHVREQSLVTDQLSRRLIRTYQLYSRTSGKHVQVLANK RINAMAEDGDPFAKLIVETDTFGSRVRVRGAETGLYICMNKKGKLIAKSN GKGKDCVFTEIVLENNYTALQNAKYEGWYMAFTRKGRPRKGSKTRQHQRE VHFMKRLPRGHHTTEQSLRFEFLNYPPFTR SLRGSQRTWA PEPR -
Predicted molecular weight
23 kDa -
Amino acids
1 to 194 -
Additional sequence information
This is the full length protein without the signal peptide, isoform 3.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab205519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilised -
Additional notes
There is 100% homology between Human 1-194 aa and mouse Mouse 23-215 aa FGF-8.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.5
Constituents: 0.29% Sodium chloride, 0.08% Sodium phosphateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile water to 0.1mg/ml. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
General Info
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Alternative names
- AIGF
- Androgen induced growth factor
- Androgen-induced growth factor
see all -
Function
Stimulates growth of the cells in an autocrine manner. Mediates hormonal action on the growth of cancer cells. -
Involvement in disease
Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6) [MIM:612702]. Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous.
Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Developmental stage
In adults expression is restricted to the gonads. -
Cellular localization
Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant human FGF8 protein (Animal Free) (ab205519)Functional analysis of ab205519 -
SDS-PAGE - Recombinant human FGF8 protein (Animal Free) (ab205519)SDS PAGE analysis of ab205519 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.
Datasheets and documents
References
ab205519 has not yet been referenced specifically in any publications.
