Bioactive grade

Recombinant human FGF8 protein (Animal Free) (ab205519)

Description

  • Product name

    Recombinant human FGF8 protein (Animal Free)
    See all FGF8 proteins and peptides
  • Biological activity

    The activity is determined by its ability to induce proliferation of mouse 3T3 cells and is typically less than 20 ng/mL. This corresponds to an expected specific activity of 5 x 104 units/mg.

  • Purity

    > 97 % SDS-PAGE.

  • Endotoxin level

    < 0.050 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    Yes
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MQVTVQSSPNFTQHVREQSLVTDQLSRRLIRTYQLYSRTSGKHVQVLANK RINAMAEDGDPFAKLIVETDTFGSRVRVRGAETGLYICMNKKGKLIAKSN GKGKDCVFTEIVLENNYTALQNAKYEGWYMAFTRKGRPRKGSKTRQHQRE VHFMKRLPRGHHTTEQSLRFEFLNYPPFTR SLRGSQRTWA PEPR
    • Predicted molecular weight

      23 kDa
    • Amino acids

      1 to 194
    • Additional sequence information

      This is the full length protein without the signal peptide, isoform 3.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab205519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Additional notes

    There is 100% homology between Human 1-194 aa and mouse Mouse 23-215 aa FGF-8. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.5
    Constituents: 0.29% Sodium chloride, 0.08% Sodium phosphate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile water to 0.1mg/ml. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

General Info

  • Alternative names

    • AIGF
    • Androgen induced growth factor
    • Androgen-induced growth factor
    • FGF 8
    • FGF-8
    • FGF-8b
    • FGF8
    • FGF8_HUMAN
    • Fibroblast growth factor 8
    • Fibroblast growth factor 8 (androgen induced)
    • Fibroblast growth factor 8 precursor
    • HBGF 8
    • HBGF-8
    • HBGF8
    • Heparin-binding growth factor 8
    • HH6
    • KAL6
    see all
  • Function

    Stimulates growth of the cells in an autocrine manner. Mediates hormonal action on the growth of cancer cells.
  • Involvement in disease

    Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6) [MIM:612702]. Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous.
    Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
  • Sequence similarities

    Belongs to the heparin-binding growth factors family.
  • Developmental stage

    In adults expression is restricted to the gonads.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • Functional analysis of ab205519
  •  SDS PAGE analysis of ab205519 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.

References

ab205519 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab205519.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up