Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 75% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human FGFR4 protein
See all FGFR4 proteins and peptides
Specific Activity: 50 pmole/min/µg
Assay Conditions: Reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA and 1 mM DTT) containing 20 µM ATP and 0.2 mg/ml poly-(Glu-Tyr) for 30 min at 30 °C.
Purity>= 75 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
Predicted molecular weight65 kDa including tags
Amino acids460 to 802
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab135023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.12% Glutathione, 0.02% Potassium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.63% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- CD 334
- CD334 antigen
FunctionReceptor for acidic fibroblast growth factor. Does not bind to basic fibroblast growth factor. Binds FGF19.
Tissue specificityExpressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
Phosphorylated on tyrosine residue (By similarity). Phosphorylation requires the presence of a functional (phosphorylated) FGFR1 and not necessarily by means of FGFR heterodimerization.
Cellular localizationMembrane. Isoform 2 may be secreted.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab135023 has not yet been referenced specifically in any publications.