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Recombinant Human FGFR4 protein (His tag) (ab167751)

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SDS-PAGE - Recombinant human FGFR4 protein (ab167751)

    Key features and details

    • Expression system: HEK 293 cells
    • Purity: > 95% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Tags: His tag C-Terminus
    • Suitable for: SDS-PAGE

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    Description

    • Product name

      Recombinant Human FGFR4 protein (His tag)
      See all FGFR4 proteins and peptides
    • Purity

      > 95 % SDS-PAGE.
      ab167751 is lyophilised from 0.22 µm filtered solution.
    • Endotoxin level

      < 1.000 Eu/µg
    • Expression system

      HEK 293 cells
    • Accession

      P22455
    • Protein length

      Protein fragment
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        LEASEEVELEPCLAPSLEQQEQELTVALGQPVRLCCGRAERGGHWYKEGS RLAPAGRVRGWRGRLEIASFLPEDAGRYLLARGSMIVLQNLTLITGDSLT SSNDDEDPKSHRDPSNRHSYPQQAPYWTHPQRMEKKLHAVPAGNTVKFRC PAAGNPTPTIRWLKDGQAFHGENRIGGIRLRHQHWSLVMESVVPSDRGTY TCLVENAVGSIRYNYLLDVLERSPHRPILQAGLPANTTAVVGSDVELLCK VYSDAQPHIQWLKHIVINGSSFGADGFPYVQVLKTADINSSEVEVLYLRN VSAEDAGEYTCLAGNSIGLSYQSAWLTVLPEEDPTWTAAAPEARYTD
      • Predicted molecular weight

        39 kDa including tags
      • Amino acids

        22 to 369
      • Tags

        His tag C-Terminus

    Associated products

    • Related Products

      • Anti-FGFR4 antibody (ab119378)
      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab167751 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Additional notes

      This product is stable after storage at:

      • -20°C to -70°C for 12 months in lyophilized state;
      • -70°C for 3 months under sterile conditions after reconstitution.
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituent: PBS

      5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%.

    • Reconstitution
      Reconstitute with sterile deionized water to a concentration of 100 µg/ml.

    General Info

    • Alternative names

      • CD 334
      • CD334
      • CD334 antigen
      • fc13h 10
      • fc13h10
      • Fgfr 4
      • FGFR-4
      • Fgfr4
      • FGFR4_HUMAN
      • Fibroblast growth factor receptor 4
      • Hydroxyaryl protein kinase
      • JTK 2
      • JTK2
      • MGC20292
      • Protein tyrosine kinase
      • TKF
      • Tyrosine kinase related to fibroblast growth factor receptor
      • Tyrosylprotein kinase
      see all
    • Function

      Receptor for acidic fibroblast growth factor. Does not bind to basic fibroblast growth factor. Binds FGF19.
    • Tissue specificity

      Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Glycosylated.
      Phosphorylated on tyrosine residue (By similarity). Phosphorylation requires the presence of a functional (phosphorylated) FGFR1 and not necessarily by means of FGFR heterodimerization.
    • Cellular localization

      Membrane. Isoform 2 may be secreted.
    • Target information above from: UniProt accession P22455 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant human FGFR4 protein (ab167751)
      SDS-PAGE - Recombinant human FGFR4 protein (ab167751)

      SDS-PAGE of reduced ab167751 stained overnight with Coomassie Blue. The protein migrates as 60 kDa due to glycosylation.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab167751? Please let us know so that we can cite the reference in this datasheet.

    ab167751 has not yet been referenced specifically in any publications.

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