Description

  • Product name

    Recombinant human FIST protein
  • Biological activity

    The Specific activity of ab85601 was determined to be 48 nmol/min/mg.
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Amino acids

      163 to 562

Specifications

Our Abpromise guarantee covers the use of ab85601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

  • Form

    Liquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

    Previously labelled as HIPK3.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.00
    Preservative: 1.02% Imidazole
    Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00308% DTT, 25% Glycerol, 1.74% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Androgen receptor interacting nuclear protein kinase
    • Androgen receptor-interacting nuclear protein kinase
    • ANPK
    • DYRK6
    • Fas interacting serine/threonine protein kinase
    • Fas-interacting serine/threonine-protein kinase
    • FIST
    • FIST3
    • Hipk3
    • HIPK3_HUMAN
    • homeodomain interacting protein kinase 3
    • Homeodomain-interacting protein kinase 3
    • Homolog of protein kinase YAK1
    • PKY
    • RP24 297H17.5
    • YAK1
    see all
  • Function

    Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors.
  • Tissue specificity

    Overexpressed in multidrug resistant cells. Highly expressed in heart and skeletal muscle, and at lower levels in placenta, pancreas, brain, spleen, prostate, thymus, testis, small intestine, colon and leukocytes. Not found in liver and lung.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated, but autophosphorylation is not required for catalytic activity.
    May be sumoylated.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

Images

  • The Specific activity of ab85601 was determined to be 48 nmol/min/mg.
  • SDS-PAGE showing ab85601 at approximately 49kDa.

References

ab85601 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab85601.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up