Recombinant human FKBP25 protein (ab93683)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MAAAVPQRAWTVEQLRSEQLPKKDIIKFLQEHGSDSFLAEHKLLGNIKNV AKTANKDHLVTAYNHLFETKRFKGTESISKVSEQVKNVKLNEDKPKETKS EETLDEGPPKYTKSVLKKGDKTNFPKKGDVVHCWYTGTLQDGTVFDTNIQ TSAKKKKNAKPSFKVGVGKVIRGWDEALLTMSKGEKARLEIEPEWAYGKK GQPDAKIPPNAKLTFEVELVDID

Specifications

Our Abpromise guarantee covers the use of ab93683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Biological activity: Specific activity is > 490 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.

    Activity Assay

    1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
    2. Add 10 µl of recombinant FKBP3 protein with 1 µg in assay buffer.
    3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
    4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
    5. Record the increase in A405 nm for 30 minutes at 25°C.
  • Applications

    Functional Studies

    SDS-PAGE

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 90 % SDS-PAGE.
    ab93683 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 25 kDa FK506-binding protein
    • 25 kDa FKBP
    • FK506 binding protein 25 T cell
    • FK506 binding protein 25, T cell, 25-KD
    • FK506 binding protein 3 (25kD)
    • FK506-binding protein 3
    • FKBP 25
    • FKBP 3
    • FKBP-25
    • FKBP-3
    • FKBP25
    • FKBP3
    • FKBP3_HUMAN
    • Immunophilin FKBP25
    • Peptidyl-prolyl cis-trans isomerase FKBP3
    • PPIase
    • PPIase FKBP3
    • Rapamycin binding protein
    • Rapamycin-selective 25 kDa immunophilin
    • Rotamase
    see all
  • Function
    FK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. PPIases accelerate the folding of proteins.
  • Sequence similarities
    Belongs to the FKBP-type PPIase family.
    Contains 1 PPIase FKBP-type domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human FKBP25 protein (ab93683)
    SDS-PAGE - Recombinant human FKBP25 protein (ab93683)
    15% SDS-PAGE showing ab93683 at approximately 25 kDa (3 µg).

References

ab93683 has not yet been referenced specifically in any publications.

Publishing research using ab93683? Please let us know so that we can cite the reference in this datasheet.

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