Product nameRecombinant human FKBP52 protein
See all FKBP52 proteins and peptides
Biological activitySpecific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris HCl pH 8.0 using chymotrypsin.
Purity> 90 % SDS-PAGE.
Endotoxin level< 1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMGSSHHHHHH SSGLVPRGSH MTAEEMKATE SGAQSAPLPM EGVDISPKQD EGVLKVIKRE GTGTEMPMIG DRVFVHYTGW LLDGTKFDSS LDRKDKFSFD LGKGEVIKAW DIAIATMKVG EVCHITCKPE YAYGSAGSPP KIPPNATLVF EVELFEFKGE DLTEEEDGGI IRRIQTRGEG YAKPNEGAIV EVALEGYYKD KLFDQRELRF EIGEGENLDL PYGLERAIQR MEKGEHSIVY LKPSYAFGSV GKEKFQIPPN AELKYELHLK SFEKAKESWE MNSEEKLEQS TIVKERGTVY FKEGKYKQAL LQYKKIVSWL EYESSFSNEE AQKAQALRLA SHLNLAMCHL KLQAFSAAIE SCNKALELDS NNEKGLFRRG EAHLAVNDFE LARADFQKVL QLYPNNKAAK TQLAVCQQRI RRQLAREKKL YANMFERLAE EENKAKAEAS SGDHPTDTEM KEEQKSNTAG SQSQVETEA
Predicted molecular weight54 kDa including tags
Amino acids1 to 459
TagsHis tag N-Terminus
- Anti-FKBP52 antibody (ab110191)
- Anti-FKBP52 antibody [EPR6619] (ab124906)
- Anti-FKBP52 antibody [EPR6618] (ab129097)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-FKBP52 antibody [Hi52C] (ab59460)
- Anti-FKBP52 antibody (ab84536)
- Anti-6X His tag® antibody (ab9108)
- Anti-FKBP52 antibody (ab97306)
Our Abpromise guarantee covers the use of ab167973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.32% Tris HCl, 10% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- 51 kDa FK506-binding protein
- 52 kDa FK506 binding protein
- 52 kDa FK506-binding protein
FunctionImmunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.
Tissue specificityWidely expressed.
Sequence similaritiesContains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats.
DomainThe PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400.
modificationsPhosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab167973 has not yet been referenced specifically in any publications.