Description

  • Product name

    Recombinant human FKBP52 protein
    See all FKBP52 proteins and peptides
  • Biological activity

    Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH 8.0 using chymotrypsin.

    Activity Assay

    1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
    2. Add 10 µl of recombinant FKBP4 protein with 1 µg in assay buffer.
    3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
    4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
    5. Record the increase in A405 nm for 30 minutes at 25°C.
  • Purity

    > 95 % SDS-PAGE.
    ab84189 is purified using conventional chromatography techniques. Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method).
  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MTAEEMKATE SGAQSAPLPM EGVDISPKQD EGVLKVIKRE GTGTEMPMIG DRVFVHYTGW LLDGTKFDSS LDRKDKFSFD LGKGEVIKAW DIAIATMKVG EVCHITCKPE YAYGSAGSPP KIPPNATLVF EVELFEFKGE DLTEEEDGGI IRRIQTRGEG YAKPNEGAIV EVALEGYYKD KLFDQRELRF EIGEGENLDL PYGLERAIQR MEKGEHSIVY LKPSYAFGSV GKEKFQIPPN AELKYELHLK SFEKAKESWE MNSEEKLEQS TIVKERGTVY FKEGKYKQAL LQYKKIVSWL EYESSFSNEE AQKAQALRLA SHLNLAMCHL KLQAFSAAIE SCNKALELDS NNEKGLFRRG EAHLAVNDFE LARADFQKVL QLYPNNKAAK TQLAVCQQRI RRQLAREKKL YANMFERLAE EENKAKAEAS SGDHPTDTEM KEEQKSNTAG SQSQVETEA

Specifications

Our Abpromise guarantee covers the use of ab84189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

    Western blot

  • Mass spectrometry

    MALDI-TOF
  • Form

    Liquid
  • Additional notes

    Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.242% Tris, 10% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • 51 kDa FK506-binding protein
    • 52 kDa FK506 binding protein
    • 52 kDa FK506-binding protein
    • 52 kDa FKBP
    • 59 kDa immunophilin
    • FK506 binding protein 4
    • FK506-binding protein 4
    • FKBP 4
    • FKBP 52
    • FKBP 59
    • FKBP-4
    • FKBP-52
    • FKBP4
    • FKBP4_HUMAN
    • FKBP51
    • FKBP52 protein
    • FKBP59
    • HBI
    • Hsp 56
    • HSP binding immunophilin
    • HSP-binding immunophilin
    • Hsp56
    • Immunophilin FKBP52
    • N-terminally processed
    • p52
    • p59
    • p59 protein
    • Peptidyl prolyl cis trans isomerase
    • peptidyl-prolyl cis-trans isomerase
    • Peptidyl-prolyl cis-trans isomerase FKBP4
    • Peptidylprolyl cis trans isomerase
    • PPIase
    • PPIase FKBP4
    • Rotamase
    • T cell FK506 binding protein 59kD
    see all
  • Function

    Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Contains 2 PPIase FKBP-type domains.
    Contains 3 TPR repeats.
  • Domain

    The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
    The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
    The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400.
  • Post-translational
    modifications

    Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab84189 at approximately 53.9kDa (3µg).

References

ab84189 has not yet been referenced specifically in any publications.

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