Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human Flt3 / CD135 protein (L610_E611insCSSDNEYFYVDFREYEYDLKWEF-PRENL) (Active)
See all Flt3 / CD135 proteins and peptides
The specific activity of FLT3-ITD-NPOS was determined to be 12 nmol/min/mg in a kinase assay using MBP substrate.
Purity> 70 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
Molecular weight informationSDS-PAGE molecular weight: ~82kDa
Amino acids571 to 993
TagsGST tag N-Terminus
Additional sequence informationThe Flt3 / CD135 mutant has 28 aa CSSDNEYFYVDFREYEYDLKWEF-PRENL insertion between 610aa and 611aa. GenBank: NM_004119
DescriptionRecombinant human Flt3 / CD135 protein (Active)
Our Abpromise guarantee covers the use of ab268586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- CD 135
- CD135 antigen
FunctionReceptor for the FL cytokine. Has a tyrosine-protein kinase activity.
Tissue specificityBone marrow cells.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 protein kinase domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268586 has not yet been referenced specifically in any publications.