Recombinant human Flt3 ligand/Flt3L protein (ab9690)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Molecular weight
      26 kDa

Associated products

Specifications

Our Abpromise guarantee covers the use of ab9690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level
    < 0.100 Eu/µg
  • Form
    Lyophilised
  • Additional notes
    The ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg.

    Previously labelled as Flt3 ligand.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with distilled water to achieve desired concentration.

General Info

  • Alternative names
    • FL
    • Flt 3 ligand
    • Flt 3L
    • Flt3 L
    • FLT3 LG
    • Flt3 ligand
    • Flt3L
    • FLT3L_HUMAN
    • Flt3lg
    • Fms related tyrosine kinase 3 ligand
    • Fms-related tyrosine kinase 3 ligand
    • SL cytokine
    see all
  • Function
    Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt

References

ab9690 has not yet been referenced specifically in any publications.

Publishing research using ab9690? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Good morning I've bought FLT3-ligand (Ab9690) but in the data sheet there is not suggestion about the reconstitution of the lyophilized protein. What is the best buffer? and the best concentration? Lot: 49746

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Answer

Thank you for your enquiry. The reconstitution instructions for this lot. Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use. We hope this helps. Thanks,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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