Recombinant human Flt3 ligand/Flt3L protein (ab9690)

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Key features and details

  • Expression system: Escherichia coli
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human Flt3 ligand/Flt3L protein
    See all Flt3 ligand/Flt3L proteins and peptides

  • Biological activity

    The ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

  • Expression system

    Escherichia coli

  • Accession

    P49771

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA

    • Predicted molecular weight

      18 kDa

Associated products

Specifications

Our Abpromise guarantee covers the use of ab9690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilized

  • Additional notes

    The ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg.

    Previously labelled as Flt3 ligand.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with distilled water to achieve desired concentration.

General Info

  • Alternative names

    • FL
    • Flt 3 ligand
    • Flt 3L
    • Flt3 L
    • FLT3 LG
    • Flt3 ligand
    • Flt3L
    • FLT3L_HUMAN
    • Flt3lg
    • Fms related tyrosine kinase 3 ligand
    • Fms-related tyrosine kinase 3 ligand
    • SL cytokine
    see all

  • Function

    Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab9690? Please let us know so that we can cite the reference in this datasheet.

ab9690 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Good morning I've bought FLT3-ligand (Ab9690) but in the data sheet there is not suggestion about the reconstitution of the lyophilized protein. What is the best buffer? and the best concentration? Lot: 49746

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Answer

Thank you for your enquiry. The reconstitution instructions for this lot. Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use. We hope this helps. Thanks,

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