Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, HPLC
Product nameRecombinant human Flt3 ligand/Flt3L protein (Animal Free)
See all Flt3 ligand/Flt3L proteins and peptides
OCI-AML5 cell proliferation: ED50 ≤10ng/ml; ≥ 1.0 x 105 units/mg.
Purity>= 98 % SDS-PAGE.
Also determined by HPCL and spectroscopy at 280 nm.
Endotoxin level<=1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthProtein fragment
SequenceMTQDCSFQHSPISSDFAVKIRELSDYLLQDYPVTVASNLQDEELCGGLWR LVLAQRWMERLKTVAGSKMQGLLERVNTEIHFVTKCAFQPPPSCLRFVQT NISRLLQETSEQLVALKPWITRQNFSRCLELQCQPDSSTLPPPWSPRPLE ATAPT
Predicted molecular weight18 kDa
Amino acids27 to 180
Additional sequence informationExtracellular domain
Our Abpromise guarantee covers the use of ab179623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product is produced with no animal-derived raw products, animal free equipment and animal free protocols.
Previously labelled as Flt3 ligand.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C long term. Avoid freeze / thaw cycle.
Constituents: 0.16% Sodium phosphate, 0.29% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionCentrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
- Flt 3 ligand
- Flt 3L
FunctionStimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab179623 has not yet been referenced specifically in any publications.