Bioactive grade

Recombinant human Flt3 ligand/Flt3L protein (Animal Free) (ab179623)

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Description

  • Product name

    Recombinant human Flt3 ligand/Flt3L protein (Animal Free)
    See all Flt3 ligand/Flt3L proteins and peptides

  • Biological activity

    OCI-AML5 cell proliferation: ED50 ≤10ng/ml; ≥ 1.0 x 105 units/mg.

  • Purity

    >= 98 % SDS-PAGE.
    Also determined by HPCL and spectroscopy at 280 nm.

  • Endotoxin level

    <=1.000 Eu/µg

  • Expression system

    Escherichia coli

  • Accession

    P49771

  • Protein length

    Protein fragment

  • Animal free

    Yes

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MTQDCSFQHSPISSDFAVKIRELSDYLLQDYPVTVASNLQDEELCGGLWR LVLAQRWMERLKTVAGSKMQGLLERVNTEIHFVTKCAFQPPPSCLRFVQT NISRLLQETSEQLVALKPWITRQNFSRCLELQCQPDSSTLPPPWSPRPLE ATAPT

    • Predicted molecular weight

      18 kDa

    • Amino acids

      27 to 180

    • Additional sequence information

      Extracellular domain

Specifications

Our Abpromise guarantee covers the use of ab179623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    HPLC

  • Form

    Lyophilised

  • Additional notes

    This product is produced with no animal-derived raw products, animal free equipment and animal free protocols.

    Previously labelled as Flt3 ligand.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.16% Sodium phosphate, 0.29% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.

General Info

  • Alternative names

    • FL
    • Flt 3 ligand
    • Flt 3L
    • Flt3 L
    • FLT3 LG
    • Flt3 ligand
    • Flt3L
    • FLT3L_HUMAN
    • Flt3lg
    • Fms related tyrosine kinase 3 ligand
    • Fms-related tyrosine kinase 3 ligand
    • SL cytokine
    see all

  • Function

    Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human Flt3 ligand/Flt3L protein (Animal Free) (ab179623)
    Functional Studies - Recombinant human Flt3 ligand/Flt3L protein (Animal Free) (ab179623)
    Functional analysis of ab179623
  • SDS-PAGE - Recombinant human Flt3 ligand/Flt3L protein (Animal Free) (ab179623)
    SDS-PAGE - Recombinant human Flt3 ligand/Flt3L protein (Animal Free) (ab179623)

     SDS PAGE analysis of ab179623 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.

References

ab179623 has not yet been referenced specifically in any publications.

Publishing research using ab179623? Please let us know so that we can cite the reference in this datasheet.

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