Recombinant Human Follistatin protein (ab113866)

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SDS-PAGE - Recombinant Human Follistatin protein (ab113866)

    Key features and details

    • Expression system: Escherichia coli
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, WB, ELISA

    Description

    • Product name

      Recombinant Human Follistatin protein
      See all Follistatin proteins and peptides

    • Expression system

      Escherichia coli

    • Accession

      P19883-1

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MKHHHHHHAS GNCWLRQAKN GRCQVLYKTE LSKEECCSTG RLSTSWTEED VNDNTLFKWM IFNGGAPNCI PCKETCENVD CGPGKKCRMN KKNKPRCVCA PDCSNITWKG PVCGLDGKTY RNECALLKAR CKEQPELEVQ YQGRCKKTCR DVFCPGSSTC VVDQTNNAYC VTCNRICPEP ASSEQYLCGN DGVTYSSACH LRKATCLLGR SIGLAYEGKC IKAKSCEDIQ CTGGKKCLWD FKVGRGRCSL CDELCPDSKS DEPVCASDNA TYASECAMKE AACSSGVLLE VKHSGSCNSI SEDTEEEEED EDQDYSFPIS SILEW

      • Predicted molecular weight

        36 kDa

      • Amino acids

        30 to 344

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab113866 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Western blot

      ELISA

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      Constituents: 0.24% Tris, 0.116% Sodium chloride

    • Reconstitution
      Add deionized water (200 µl) to prepare a working stock solution of approximately 0.5 mg/mL

    General Info

    • Alternative names

      • Activin binding protein
      • Activin-binding protein
      • Follistatin
      • FS
      • fst
      • FST_HUMAN
      • Human follistatin gene
      see all

    • Function

      Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).

    • Tissue specificity

      Isoform 1 is the predominant isoform in serum but is undetectable in follicular fluid.

    • Sequence similarities

      Contains 3 follistatin-like domains.
      Contains 3 Kazal-like domains.
      Contains 1 TB (TGF-beta binding) domain.

    • Cellular localization

      Secreted.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Follistatin protein (ab113866)
      SDS-PAGE - Recombinant Human Follistatin protein (ab113866)
      14% SDS-PAGE separation of Human Follistatin
      1. reduced and boiled sample, 5μg/lane
      2. non-reduced and non-boiled sample, 5μg/lane

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab113866? Please let us know so that we can cite the reference in this datasheet.

    ab113866 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    Please advise regarding Follistatin peptide,

    We have a customer who looking after 2mg concentrated peptide. What is your recommendation referring to the following conditions?



    Introduction:
    Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (fsh).

    Description:
    Follistatin Human Recombinant produced in is a single, non-glycosylated polypeptide chain containing 288 amino acids and having a total molecular mass of 31.5kDa.
    The FST is purified by proprietary chromatographic techniques.

    Source:
    Sterile Filtered White lyophilized (freeze-dried) powder.

    Stability:
    Lyophilized Follistatin although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution FST should be stored at 4C between 2-7 days and for future use below -18C.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please prevent freeze-thaw cycles.

    Purity:
    Greater than 95.0% as determined by
    (a) Analysis by RP-HPLC.
    (b) Analysis by SDS-PAGE.



    Please let us know which Abcam item is recommended.

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    Answer

    We have two products which may be suitable for your customer as the concentration of these two can be influenced on how much water is added to the lyophilised protein:

    ab50163 Follistatin protein

    https://www.abcam.com/Recombinant-human-Follistatin-protein-ab50163.html (or use the following: https://www.abcam.com/Recombinant-human-Follistatin-protein-ab50163.html).



    ab113866 Follistatin protein

    https://www.abcam.com/Recombinant-Human-Follistatin-protein-ab113866.html (or use the following: https://www.abcam.com/Recombinant-Human-Follistatin-protein-ab113866.html).



    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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