Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      GNCWLRQAKN GRCQVLYKTE LSKEECCSTG RLSTSWTEED VNDNTLFKWM IFNGGAPNCI PCKETCENVD CGPGKKCRMN KKNKPRCVCA PDCSNITWKG PVCGLDGKTY RNECALLKAR CKEQPELEVQ YQGRCKKTCR DVFCPGSSTC VVDQTNNAYC VTCNRICPEP ASSEQYLCGN DGVTYSSACH LRKATCLLGR SIGLAYEGKC IKAKSCEDIQ CTGGKKCLWD FKVGRGRCSL CDELCPDSKS DEPVCASDNA TYASECAMKE AACSSGVLLE VKHSGSCN
    • Molecular weight
      32 kDa
    • Amino acids
      30 to 317

Specifications

Our Abpromise guarantee covers the use of ab50163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Determined by its ability to neutralize Activin A inhibitory effect of murine MPC-11 cells. The expected ED50 is 0.1-0.4 µg/ml in the presence of 7.5 ng/ml Activin A.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 95 % SDS-PAGE.
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Activin binding protein
    • Activin-binding protein
    • Follistatin
    • FS
    • fst
    • FST_HUMAN
    • Human follistatin gene
    see all
  • Function
    Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).
  • Tissue specificity
    Isoform 1 is the predominant isoform in serum but is undetectable in follicular fluid.
  • Sequence similarities
    Contains 3 follistatin-like domains.
    Contains 3 Kazal-like domains.
    Contains 1 TB (TGF-beta binding) domain.
  • Cellular localization
    Secreted.
  • Information by UniProt

References

ab50163 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question

Please advise regarding Follistatin peptide,

We have a customer who looking after 2mg concentrated peptide. What is your recommendation referring to the following conditions?



Introduction:
Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (fsh).

Description:
Follistatin Human Recombinant produced in is a single, non-glycosylated polypeptide chain containing 288 amino acids and having a total molecular mass of 31.5kDa.
The FST is purified by proprietary chromatographic techniques.

Source:
Sterile Filtered White lyophilized (freeze-dried) powder.

Stability:
Lyophilized Follistatin although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution FST should be stored at 4C between 2-7 days and for future use below -18C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 95.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.



Please let us know which Abcam item is recommended.

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Answer

We have two products which may be suitable for your customer as the concentration of these two can be influenced on how much water is added to the lyophilised protein:

ab50163 Follistatin protein

https://www.abcam.com/index.html?datasheet=50163 (or use the following: https://www.abcam.com/index.html?datasheet=50163).



ab113866 Follistatin protein

https://www.abcam.com/index.html?datasheet=113866 (or use the following: https://www.abcam.com/index.html?datasheet=113866).



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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