For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Please advise regarding Follistatin peptide,
We have a customer who looking after 2mg concentrated peptide. What is your recommendation referring to the following conditions?
Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (fsh).
Follistatin Human Recombinant produced in is a single, non-glycosylated polypeptide chain containing 288 amino acids and having a total molecular mass of 31.5kDa.
The FST is purified by proprietary chromatographic techniques.
Sterile Filtered White lyophilized (freeze-dried) powder.
Lyophilized Follistatin although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution FST should be stored at 4C between 2-7 days and for future use below -18C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Greater than 95.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Please let us know which Abcam item is recommended.
Asked on Jul 10 2012
We have two products which may be suitable for your customer as the concentration of these two can be influenced on how much water is added to the lyophilised protein:
ab50163 Follistatin protein
https://www.abcam.com/Recombinant-human-Follistatin-protein-ab50163.html (or use the following: https://www.abcam.com/Recombinant-human-Follistatin-protein-ab50163.html).
ab113866 Follistatin protein
https://www.abcam.com/Recombinant-Human-Follistatin-protein-ab113866.html (or use the following: https://www.abcam.com/Recombinant-Human-Follistatin-protein-ab113866.html).
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Jul 10 2012