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Signal Transduction Signaling Pathway G Protein Signaling GPCR
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Recombinant Human Frizzled 4 protein (Fc Chimera) (ab219656)

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SDS-PAGE - Recombinant Human Frizzled 4 protein (Fc Chimera) (ab219656)

    Key features and details

    • Expression system: HEK 293 cells
    • Purity: > 95% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human Frizzled 4 protein (Fc Chimera)
      See all Frizzled 4 proteins and peptides
    • Purity

      > 95 % SDS-PAGE.

    • Endotoxin level

      < 1.000 Eu/µg
    • Expression system

      HEK 293 cells
    • Accession

      Q9ULV1
    • Protein length

      Protein fragment
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        FGDE EERRCDPIRI SMCQNLGYNV TKMPNLVGHE LQTDAELQLT TFTPLIQYGC SSQLQFFLCS VYVPMCTEKI NIPIGPCGGM CLSVKRRCEP VLKEFGFAWP ESLNCSKFPP QNDHNHMCME GPGDEEVPLP HKTPIQPGEE
      • Predicted molecular weight

        43 kDa including tags
      • Amino acids

        37 to 180
      • Additional sequence information

        Human IgG1 Fc tag at the C-terminus.

    Associated products

    • Related Products

      • Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)

    Specifications

    Our Abpromise guarantee covers the use of ab219656 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

      pH: 7.40
      Constituents: 5% Trehalose, PBS

    • Reconstitution
      Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

    General Info

    • Alternative names

      • CD 344
      • CD 344 antigen
      • CD344
      • CD344 antigen
      • EVR1
      • FEVR
      • Frizzled (Drosophila) homolog 4
      • Frizzled 4
      • Frizzled family receptor 4
      • Frizzled homolog 4 (Drosophila)
      • Frizzled-4
      • Fz 4
      • Fz-4
      • Fz4
      • FZD4
      • FZD4_HUMAN
      • FZD4S
      • FzE4
      • GPCR
      • hFz4
      • MGC34390
      • WNT receptor frizzled 4
      see all
    • Function

      Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
    • Tissue specificity

      Almost ubiquitous. Largely expressed in adult heart, skeletal muscle, ovary, and fetal kidney. Moderate amounts in adult liver, kidney, pancreas, spleen, and fetal lung, and small amounts in placenta, adult lung, prostate, testis, colon, fetal brain and liver.
    • Involvement in disease

      Defects in FZD4 are the cause of vitreoretinopathy exudative type 1 (EVR1) [MIM:133780]; also known as autosomal dominant familial exudative vitreoretinopathy (FEVR) or Criswick-Schepens syndrome. EVR1 is a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina. This may lead to compensatory retinal neovascularization, which is thought to be induced by hypoxia from the initial avascular insult. New vessels are prone to leakage and rupture causing exudates and bleeding, followed by scarring, retinal detachment and blindness. Clinical features can be highly variable, even within the same family. Patients with mild forms of the disease are asymptomatic, and their only disease-related abnormality is an arc of avascular retina in the extreme temporal periphery.
    • Sequence similarities

      Belongs to the G-protein coupled receptor Fz/Smo family.
      Contains 1 FZ (frizzled) domain.
    • Domain

      Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
      The FZ domain is involved in binding with Wnt ligands.
    • Cellular localization

      Membrane. Cell membrane.
    • Target information above from: UniProt accession Q9ULV1 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Frizzled 4 protein (Fc Chimera) (ab219656)
      SDS-PAGE - Recombinant Human Frizzled 4 protein (Fc Chimera) (ab219656)

      SDS-PAGE using ab219656. DTT-reduced protein migrates as 50-65 kDa due to glycosylation.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab219656? Please let us know so that we can cite the reference in this datasheet.

    ab219656 has not yet been referenced specifically in any publications.

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