Recombinant Human Frizzled 7 protein (ab191958)

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Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
      O75084
    • Species
      Human
    • Sequence
      QPYHGEKGISVPDHGFCQPISIPLCTDIAYNQTILPNLLGHTNQEDAGLE VHQFYPLVKVQCSPELRFFLCSMYAPVCTVLDQAIPPCRSLCERARQGCE ALMNKFGFQWPERLRCENFPVHGAGEICVGQNTSDGSGGPGGGPTAYPTA PYL
    • Molecular weight
      43 kDa including tags
    • Amino acids
      33 to 185
    • Additional sequence information
      Fused with Fc fragment of Human IgG1 at the C-terminus (AAH15915).

Specifications

Our Abpromise guarantee covers the use of ab191958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Endotoxin level
    < 1.000 Eu/µg
  • Purity
    > 92 % SDS-PAGE.

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C prior to reconstitution. Upon reconsitution add a carrier protein (0.1% BSA). Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 5% Trehalose, 0.75% Glycine, 0.61% Tris

    Lyophilized from 0.22 µm filtered solution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names
    • Frizzled 7, seven transmembrane spanning receptor
    • frizzled class receptor 7
    • Frizzled drosophila homolog of 7
    • Frizzled family receptor 7
    • Frizzled homolog 7
    • Frizzled-7
    • Frizzled7
    • Fz 7
    • Fz-7
    • Fz7
    • FZD 7
    • FZD7
    • FZD7_HUMAN
    • FzE 3
    • FzE3
    • hFz 7
    • hFz7
    • Homolog of Drosophila Frizzled 7
    • OTTHUMP00000163742
    see all
  • Function
    Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • Tissue specificity
    High expression in adult skeletal muscle and fetal kidney, followed by fetal lung, adult heart, brain, and placenta. Specifically expressed in squamous cell esophageal carcinomas.
  • Sequence similarities
    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • Domain
    Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localization
    Membrane.
  • Information by UniProt

Images

  • SDS-PAGE - Human Frizzled 7 protein fragment (ab191958)
    SDS-PAGE - Human Frizzled 7 protein fragment (ab191958)

    SDS-PAGE analysis of DTT-reduced ab191958 stained overnight with Coomassie Blue. DTT-reduced protein migrates as 50-55 kDa due to glycosylation.

References

ab191958 has not yet been referenced specifically in any publications.

Publishing research using ab191958? Please let us know so that we can cite the reference in this datasheet.

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