Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
      P42685-1
    • Species
      Human
    • Molecular weight
      60 kDa
    • Amino acids
      208 to 505
    • Tags
      GST tag N-Terminus
    • Additional sequence information
      Recombinant fragment, corresponding to amino acids 208-end of Human FRK.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab60861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific Activity: 997 nmol/min/mg.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form
    Liquid
  • Additional notes

    ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Beta cell Src homology tyrosine kinase
    • BSK
    • BSK/IYK
    • C85044
    • Frk
    • Frk fyn related kinase
    • FRK_HUMAN
    • FYN related kinase
    • FYN-related kinase
    • GASK
    • Gastrointestinal associated kinase
    • Gastrointestinal associated tyrosine kinase
    • GTK
    • GTK, mouse, homolog of
    • Intestine tyrosine kinase
    • Nuclear tyrosine protein kinase RAK
    • Protein-tyrosine kinase 5
    • PTK5
    • PTK5 protein tyrosine kinase 5
    • RAK
    • Tyrosine protein kinase FRK
    • Tyrosine-protein kinase FRK
    see all
  • Function
    Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation possibly by reducing its binding to NEDD4. May function as a tumor suppressor.
  • Tissue specificity
    Restricted to cells lines derived from tissues of lymphoid, brain, breast, colon and bladder origin.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • Functional Studies - FRK protein (Tagged) (ab60861)
    Functional Studies - FRK protein (Tagged) (ab60861)
    Sample Kinase Activity Plot.
  • SDS-PAGE - FRK protein (Tagged) (ab60861)
    SDS-PAGE - FRK protein (Tagged) (ab60861)
    ab60861 on SDS-PAGE, MW ~60 kDa.

References

ab60861 has not yet been referenced specifically in any publications.

Publishing research using ab60861? Please let us know so that we can cite the reference in this datasheet.

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