Recombinant human FRK protein (ab60861)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human FRK protein -
Biological activity
Specific Activity: 997 nmol/min/mg.
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Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
60 kDa -
Amino acids
208 to 505 -
Tags
GST tag N-Terminus -
Additional sequence information
Recombinant fragment, corresponding to amino acids 208-end of Human FRK.
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Beta cell Src homology tyrosine kinase
- BSK
- BSK/IYK
see all -
Function
Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation possibly by reducing its binding to NEDD4. May function as a tumor suppressor. -
Tissue specificity
Restricted to cells lines derived from tissues of lymphoid, brain, breast, colon and bladder origin. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
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Functional Studies - Recombinant human FRK protein (ab60861)The specific activity of FRK (ab60861) was determined to be 848 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human FRK protein (ab60861)SDS PAGE analysis of ab60861 -
SDS-PAGE - Recombinant human FRK protein (ab60861)SDS PAGE analysis of ab60861 -
Functional Studies - Recombinant human FRK protein (ab60861)Sample Kinase Activity Plot.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab60861 has not yet been referenced specifically in any publications.