Recombinant human FRK protein (ab60861)
- Datasheet
- References
- Protocols
Description
-
Product name
Recombinant human FRK protein -
Biological activity
Specific Activity: 997 nmol/min/mg.
-
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Predicted molecular weight
60 kDa -
Amino acids
208 to 505 -
Tags
GST tag N-Terminus -
Additional sequence information
Recombinant fragment, corresponding to amino acids 208-end of Human FRK.
-
Associated products
-
Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
Functional Studies
-
Form
Liquid -
Additional notes
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- Beta cell Src homology tyrosine kinase
- BSK
- BSK/IYK
see all -
Function
Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation possibly by reducing its binding to NEDD4. May function as a tumor suppressor. -
Tissue specificity
Restricted to cells lines derived from tissues of lymphoid, brain, breast, colon and bladder origin. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
-
Functional Studies - Recombinant human FRK protein (ab60861)The specific activity of FRK (ab60861) was determined to be 848 nmol/min/mg as per activity assay protocol -
SDS-PAGE - Recombinant human FRK protein (ab60861)SDS PAGE analysis of ab60861 -
SDS-PAGE - Recombinant human FRK protein (ab60861)SDS PAGE analysis of ab60861 -
Functional Studies - Recombinant human FRK protein (ab60861)Sample Kinase Activity Plot.
Datasheets and documents
References
ab60861 has not yet been referenced specifically in any publications.
