Recombinant Human PFKM protein (ab199591)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, HPLC
Description
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Product name
Recombinant Human PFKM protein
See all PFKM proteins and peptides -
Purity
> 95 % SDS-PAGE.
Determined by SEC-HPLC and reducing SDS-PAGE -
Endotoxin level
< 0.100 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
THEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFV HEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRA AYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEAT KSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQR TFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETR TRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQR GGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMEC VQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSH TVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEA GWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEA YTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICT TCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFT IRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGI FDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIF ANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKI LAKYEIDLDTSDHAHLEHITRKRSGEAAVVDHHHHHH -
Predicted molecular weight
86 kDa including tags -
Amino acids
2 to 780 -
Tags
His tag C-Terminus -
Additional sequence information
This product is for the mature full length protein. The initiator methionine is not included.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab199591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
HPLC
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Form
Liquid -
Additional notes
This product was previously labelled as Fructose 6 Phosphate Kinase
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 6.9
Constituents: 0.87% Sodium chloride, 99% Phosphate Buffer
Supplied as a 0.2 µm filtered solution
General Info
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Alternative names
- 6 Phosphofructokinase Muscle Type
- 6-phosphofructokinase
- 6-phosphofructokinase muscle type
see all -
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. -
Involvement in disease
Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7) [MIM:232800]; also known as Tarui disease. GSD7 is an autosomal recessive disorder characterized by exercise intolerance with associated nausea and vomiting. Short bursts of intense activity are particularly difficult. Severe muscle cramps and myoglobinuria develop after vigorous exercise. Most patients obtain a "second wind" when the onset of exercise is followed by a brief rest period. In time patients adjust their activity level and are well compensated. -
Sequence similarities
Belongs to the phosphofructokinase family. Two domains subfamily. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab199591 has not yet been referenced specifically in any publications.