Key features and details
- Expression system: CHO cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, HPLC
Product nameRecombinant human FSTL1/FRP protein (Active)
See all FSTL1/FRP proteins and peptides
Measured by its binding ability in a functional ELISA. When ab245801 is coated at 200 ng/well, the concentration of recombinant human BMP-4 that produces 50% of the optimal binding response is found to be approximately 250 ng/mL.
Purity> 95 % SDS-PAGE.
> 95 % by HPLC.
Expression systemCHO cells
Protein lengthFull length protein
SequenceEEELRSKSKICANVFCGAGRECAVTEKGEPTCLCIEQCKPHKRPVCGSNG KTYLNHCELHRDACLTGSKIQVDYDGHCKEKKSVSPSASPVVCYQSNRDE LRRRIIQWLEAEIIPDGWFSKGSNYSEILDKYFKNFDNGDSRLDSSEFLK FVEQNETAINITTYPDQENNKLLRGLCVDALIELSDENADWKLSFQEFLK CLNPSFNPPEKKCALEDETYADGAETEVDCNRCVCACGNWVCTAMTCDGK NQKGAQTQTEEEMTRYVQELQKHQETAEKTKRVSTKEI
Predicted molecular weight33 kDa
Amino acids21 to 308
Additional sequence informationFull length mature chain without signal peptide.
Our Abpromise guarantee covers the use of ab245801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Due to glycosylation, protein migration occurs at an apparent molecular weight of approximately 50-55 kDa by SDS-PAGE analysis under both reducing and non-reducing conditions.
This product was previously labelled as FSTL1
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 0.16% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute in water to 0.1-1.0 mg/ml.
- Anti FSTL1
FunctionMay modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin.
Tissue specificityOverexpressed in synovial tissues from rheumatoid arthritis.
Sequence similaritiesContains 2 EF-hand domains.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
Contains 1 VWFC domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245801 has not yet been referenced specifically in any publications.