Recombinant Human FTO protein (ab109039)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Tags: His tag N-Terminus
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Human FTO protein
    See all FTO proteins and peptides

  • Purity

    > 90 % SDS-PAGE.
    ab109039 is 0.2µm filtered.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    Escherichia coli

  • Accession

    Q9C0B1

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      KRTPTAEEREREAKKLRLLEELEDTWLPYLTPKDDEFYQQWQLKYPKLIL REASSVSEELHKEVQEAFLTLHKHGCLFRDLVRIQGKDLLTPVSRILIGN PGCTYKYLNTRLFTVPWPVKGSNIKHTEAEIAAACETFLKLNDYLQIETI QALEELAAKEKANEDAVPLCMSADFPRVGMGSSYNGQDEVDIKSRAAYNV TLLNFMDPQKMPYLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYSYSCEG PEEESEDDSHLEGRDPDIWHVGFKISWDIETPGLAIPLHQGDCYFMLDDL NATHQHCVLAGSQPRFSSTHRVAECSTGTLDYILQRCQLALQNVCDDVDN DDVSLKSFEPAVLKQGEEIHNEVEFEWLRQFWFQGNRYRKCTDWWCQPMA QLEALWKKMEGVTNAVLHEVKREGLPVEQRNEILTAILASLTARQNLRRE WHARCQSRIARTLPADQKPECRPYWEKDDASMPLPFDLTDIVSELRGQLL EAKP

    • Predicted molecular weight

      65 kDa including tags

    • Amino acids

      2 to 505

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab109039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 8.20
    Constituents: 0.869% Tris HCl, 0.87% Sodium chloride

General Info

  • Alternative names

    • AlkB homolog 9
    • ALKBH9
    • Alpha-ketoglutarate-dependent dioxygenase FTO
    • AW743446
    • Fat mass and obesity-associated protein
    • FATSO, MOUSE, HOMOLOG OF
    • Fto
    • FTO_HUMAN
    • GDFD
    • KIAA1752
    • mKIAA1752
    • Protein fatso
    see all

  • Function

    Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.

  • Tissue specificity

    Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.

  • Involvement in disease

    Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GRDDCFED) [MIM:612938]. The disease consists of a severe children multiple congenital anomaly syndrome with death by the age of 3 years. All affected individuals had postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits, and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate were also observed.

  • Sequence similarities

    Belongs to the fto family.

  • Domain

    The 3D-structure of the Fe2OG dioxygenase domain is similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity is very low. As a consequence, the domain is not detected by protein signature databases.

  • Cellular localization

    Nucleus.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab109039? Please let us know so that we can cite the reference in this datasheet.

ab109039 has been referenced in 1 publication.

  • Su R  et al. R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA Signaling. Cell 172:90-105.e23 (2018). PubMed: 29249359

Customer reviews and Q&As

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Question

1. Has there been any activity testing done with this protein?
2. Is it possible to cleave off the His tag? For example, is there a protease cleavage site designed for removal of the tag?

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Answer

At this time we have not determined it's enzymatic activity. Also, cleavage of the His-tag is not possible.

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