Recombinant Human Furin protein (denatured) (ab203526)

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Description

  • Product name

    Recombinant Human Furin protein (denatured)
    See all Furin proteins and peptides

  • Purity

    > 85 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    P09958

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMDVYQEPTDPKFPQ QWYLSGVTQRDLNVKAAWAQGYTGHGIVVSILDDGIEKNHPDLAGNYDPG ASFDVNDQDPDPQPRYTQMNDNRHGTRCAGEVAAVANNGVCGVGVAYNAR IGGVRMLDGEVTDAVEARSLGLNPNHIHIYSASWGPEDDGKTVDGPARLA EEAFFRGVSQGRGGLGSIFVWASGNGGREHDSCNCDGYTNSIYTLSISSA TQFGNVPWYSEACSSTLATTYSSGNQNEKQIVTTDLRQKCTESHTGTSAS APLAAGIIALTLEANKNLTWRDMQHLVVQTSKPAHLNANDWATNGVGRKV SHSYGYGLLDAGAMVALAQNWTTVAPQRKCIIDILTEPKDIGKRLEVRKT VTACLGEPNHITRLEHAQARLTLSYNRRGDLAIHLVSPMGTRSTLLAARP HDYSADGFNDWAFMTTHSWDEDPSGEWVLEIENTSEANNYGTLTKFTLVL YGTAPEGLPVPPESSGCKTLTSSQACVVCEEGFSLHQKSCVQHCPPGFAP QVLDTHYSTENDVETIRASVCAPCHASCATCQGPALTDCLSCPSHASLDP VEQTCSRQSQSSRESPPQQQPPRLPPEVEAGQRLRAGLLPSHLPE

    • Predicted molecular weight

      70 kDa including tags

    • Amino acids

      108 to 715

    • Tags

      His tag N-Terminus

    • Additional sequence information

      NP_002560.

  • Description

    Recombinant Human Furin protein

Specifications

Our Abpromise guarantee covers the use of ab203526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8
    Constituents: 0.32% Tris HCl, 10% Glycerol

General Info

  • Alternative names

    • Dibasic processing enzyme
    • Dibasic-processing enzyme
    • FES upstream region
    • FUR
    • FURIN
    • Furin membrane associated receptor protein
    • FURIN_HUMAN
    • PACE
    • Paired basic amino acid residue cleaving enzyme
    • Paired basic amino acid residue-cleaving enzyme
    • PCSK3
    • Proprotein convertase subtilisin/kexin type 3
    • SPC1
    see all

  • Function

    Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.

  • Tissue specificity

    Seems to be expressed ubiquitously.

  • Sequence similarities

    Belongs to the peptidase S8 family. Furin subfamily.
    Contains 1 homo B/P domain.

  • Domain

    Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.

  • Post-translational
    modifications

    The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
    Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms.

  • Cellular localization

    Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Furin protein (denatured) (ab203526)
    SDS-PAGE - Recombinant Human Furin protein (denatured) (ab203526)

    15% SDS-PAGE analysis of ab203526 (3 μg).

References

ab203526 has not yet been referenced specifically in any publications.

Publishing research using ab203526? Please let us know so that we can cite the reference in this datasheet.

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