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Inquiry: I would like to buy an anti-Fyn antibody made in mouse that detects fyn form human and mouse tissues. This product 1881 says it is like this but the picture of the blot shows a band higher than 59 KDa and also says it was used an anti-goat secondary antibody. could you clarify about all this?
Asked on Sep 17 2013
Thank you for your inquiry.
The image on the datasheet of ab1881 shows a WB with the positive control ab84696.
ab84696 is a Active human Fyn full length protein that has a tag. The tag has a size of ca. 26kDa.
Therefore the band at ca 85 kDa is what we would expect.
If you are using a sample with endogenous Fyn the expected size is ca 59 kDa.
I hope this information is helpful and wish you good luck with your research.
Anja HoffmannAbcam Scientific Support
Answered on Sep 17 2013