Bioactive grade

Recombinant human G-CSF protein (ab9692)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 0.100 Eu/µg
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human G-CSF protein
    See all G-CSF proteins and peptides

  • Biological activity

    The ED50, determined by the dose-dependent stimulation of the proliferation of murine NSF-60 cells, is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.

  • Purity

    > 95 % SDS-PAGE.
    Sterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses.

  • Endotoxin level

    < 0.100 Eu/µg

  • Expression system

    Escherichia coli

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      Human G-CSF is a 18.7 kDa protein containing 174 amino acid residues: TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLG IPWAPLSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG P TLDTLQLDV ADFATTIWQQMEELGMAPAL QPTQGAMPAF ASAFQRRA GG VLVASHLQSF LEVSYRVLRH LAQP

Associated products

Specifications

Our Abpromise guarantee covers the use of ab9692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilized

  • Additional notes

    The ED50 as determined by the dose-dependent stimulation of the proliferation of murine NFS-60 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    For lot specific reconstitution information please contact our Scientific Support Team.

General Info

  • Alternative names

    • C17orf33
    • Colony stimulating factor 3
    • Colony stimulating factor 3 (granulocyte)
    • CSF 3
    • CSF beta
    • CSF3
    • CSF3_HUMAN
    • CSF3OS
    • Csfg
    • Filgrastim
    • G-CSF
    • GCSA
    • GCSF
    • Granulocyte colony stimulating factor
    • Granulocyte colony-stimulating factor
    • Lenograstim
    • Macrophage granulocyte inducer 2
    • MGC45931
    • MGI 2
    • Pluripoietin
    see all

  • Function

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

  • Sequence similarities

    Belongs to the IL-6 superfamily.

  • Post-translational
    modifications

    O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

  • Cellular localization

    Secreted.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab9692? Please let us know so that we can cite the reference in this datasheet.

ab9692 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

I am interested in ordering recombinant human G-CSF from you. However,there are two products that describe the peptide as "Highly pure (>98%) recombinant hIL-11 (human Interleukin-11)". ab9752 has the recombinant murine sequence whereas ab9692 has the human sequence. Can I therefore assume that ab9692 is the human protein? Also, is IL-11 another name for G-CSF or simply an agonist of the G-CSF receptor?

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Answer

Thank you for your email. There was a mistake on the datasheets for these proteins - ab9692 is recombinant human G-CSF and ab9752 is recombinant mouse G-CSF. They are not IL-ll proteins. I have updated the online datasheets to reflect this information. So, it sounds like what you want is ab9692, the recombinant human G-CSF. If you have any additional questions, please contact us again.

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