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    recombinant-human-gaba-b-receptor-1-protein-ab112287.pdf

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Neuroscience Neurotransmitter Amino Acids GABA
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Recombinant Human GABA B Receptor 1 protein (ab112287)

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SDS-PAGE - Recombinant Human GABA B Receptor 1 protein (ab112287)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: SDS-PAGE, WB, ELISA

    Description

    • Product name

      Recombinant Human GABA B Receptor 1 protein
    • Expression system

      Wheat germ
    • Accession

      Q9UBS5-2
    • Protein length

      Protein fragment
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        AVYIGALFPMSGGWPGGQACQPAVEMALEDVNSRRDILPDYELKLIHHDS KCDPGQATKYLYELLYNDPIKIILMPGCSSVSTLVAEAARMWNLIVLSYG
      • Predicted molecular weight

        37 kDa
      • Amino acids

        52 to 151
      • Tags

        GST tag N-Terminus

    Associated products

    • Related Products

      • Anti-GABA B Receptor 1 antibody [2D7] (ab55051)

    Specifications

    Our Abpromise guarantee covers the use of ab112287 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Western blot

      ELISA

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

      Glutathione is reduced

    General Info

    • Alternative names

      • dJ271M21.1.1
      • dJ271M21.1.2
      • FLJ92613
      • GABA-B receptor 1
      • GABA-B-R1
      • GABA-BR1
      • GABAB R1
      • GABAB subunit 1c
      • GABABR1
      • GABBR1
      • GABBR1 3
      • GABR1_HUMAN
      • Gamma aminobutyric acid (GABA) B receptor 1
      • Gamma-aminobutyric acid type B receptor subunit 1
      • Gb1
      • GPRC3A
      • Seven transmembrane helix receptor
      see all
    • Function

      Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Activated by (-)-baclofen, cgp27492 and blocked by phaclofen.
      Isoform 1E function may be to regulate the availability of functional GABA-B-R1A/GABA-B-R2 heterodimers by competing for GABA-B-R2 dimerization. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.
    • Tissue specificity

      Highly expressed in brain and weakly in heart, small intestine and uterus. Isoform 1A is mostly expressed in granular cell and molecular layer. Isoform 1B is mostly expressed in Purkinje cells. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
      Contains 2 Sushi (CCP/SCR) domains.
    • Domain

      Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 2. The linker region between the transmembrane domain 3 (TM3) and the transmembrane domain 4 (TM4) probably play a role in the specificity for G-protein coupling.
    • Cellular localization

      Secreted and Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Colocalizes with ATF4 in hippocampal neuron dendritic membranes (By similarity). Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane.
    • Target information above from: UniProt accession Q9UBS5 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human GABA B Receptor 1 protein (ab112287)
      SDS-PAGE - Recombinant Human GABA B Receptor 1 protein (ab112287)
      ab112287 analysed on a 12.5% SDS-PAGE stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab112287? Please let us know so that we can cite the reference in this datasheet.

    ab112287 has not yet been referenced specifically in any publications.

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